background: species of microsporidia have been known as opportunistic obli-gate intracellular parasites particularly in immunocompromised patients. enterocyto-zoon bieneusi is one of most prevalent intestinal microsporida parasites in hiv + /aids patients. in this study, intestinal microsporidia infection was deter-mined in hiv + /aids patients using microscopic and molecular methods. methods: ...

background: the objective of the study was to evaluate the presence of neospora caninum organisms in the brain of aborted fetuses and placentas of full-term calves born of seropositive cows. methods: during 2006-2007, 12 brains of aborted calves from neospora seropositive cattle and 7 pla­centas from seropositive dams giving birth to full-term calves, from four dairy cattle farms located around...

objective :cryptosporidiosis is one of the most important parasitic infections in iran which causes diarrhea in humans and animals. the identification of the cryptosporidium species among humans is necessary. this study aims to identify species of cryptosporidium isolated from patients that referred to three hospitals in tehran based on the 18s rrna gene by nested pcr-rflp assay. ‎methods :in t...

Problem statement: Q fever is a ubiquitous zoonosis caused by Coxiella burnetii, an obligate intracellular rickettsial organism that caused abortion and stillbirth in ruminants. Approach: The prevalence of Coxiella burnetii in Iran is essentially unknown. Its traditional diagnosis is based on culture, serology and conventional PCR. In this present study, for more sensitive and accurate detectio...

For the rapid identification and detection of Vibrio anguillarum, we have PCR amplification technique targeting gyrB region has been evaluated. We have designed twosets of PCR primers for the specific amplification of gyrB in V. anguillarum using single and nested PCR. PCRs specificity was demonstrated by successful amplicon from V. anguillarum DNA. The detection limit of the single PCRs and th...

The determination of unknown DNA sequences around a known locus has important applications in molecular genetics, specifically in genomic walking and genome mapping. Several PCR-based methods have been reported to address this issue, but they often involve multiple, time-consuming steps. We have previously described a technique known as restriction site PCR (RS-PCR) that allows sequence acquisi...