The scope of paternal contributions during early embryonic development has long been considered limited. Dramatic changes in chromatin structure throughout spermatogenesis have been thought to leave the sperm void of complex layers of epigenetic regulation over the DNA blueprint, thus leaving the balance of that regulation to the oocyte. However, recent work in the fields of epigenetics and mal...

Introduction: Date palm gemmule has been used in traditional medicine to improve the semen quality and treat infertility. Their components remedies those are rich of antioxidant and vitamins can influence spermatogenesis. The aim of this project was to evaluate the effects of date palm gommule water/alcoholic extract on reproductive tissues, sex hormone and sperm quality of rat. Methods: Sev...

introduction: date palm gemmule has been used in traditional medicine to improve the semen quality and treat infertility. their components remedies those are rich of antioxidant and vitamins can influence spermatogenesis. the aim of this project was to evaluate the effects of date palm gommule water/alcoholic extract on reproductive tissues, sex hormone and sperm quality of rat. methods: sevent...

OBJECTIVE To examine levels of sperm DNA damage and oxidative stress (OS) in infertile men with varicocele. DESIGN Prospective controlled study. SETTING Male infertility clinic, Glickman Urological Institute, Cleveland Clinic Foundation, Cleveland, Ohio. PATIENT(S) Thirty-one infertility patients and 16 fertile controls. INTERVENTION(S) Sperm DNA fragmentation index (DFI), levels of sem...

introduction: semen parameters and specially sperm morphology is used for the assessment of semen sample and for the prediction of the fertilization. however there is semen samples with normal sperm morphology but with hidden sperm chromatin anomalies. since sperm morphology and chromatin anomalies reduce the fertilization rat independently, the purpose of this study is to consider the relation...

The most effective method to control the sex of offspring is by separating X- from Y-bearing sperm on the basis of their DNA content. Sperm can be stained with Hoechst 33342 and efficiently sexed using a flow cytometer/cell sorter. However, applying this established assay to cryopreserved bovine sperm presents specific problems, such as broad fluorescence distributions without a distinct X- and...

Infertility affects one in six couples in Europe during their reproductive years with dysfunctional sperm being one of the most common causes. Conventional semen analysis has proven variable and lacking in prognostic value so, over the past decade, more useful molecular fertility biomarkers have been explored. Among the tests showing most promise are those measuring sperm DNA quality. Sperm DNA...