The BioID method uses a promiscuous biotin ligase to detect protein-protein associations as well as proximate proteins in living cells. Here we report improvements to the BioID method centered on BioID2, a substantially smaller promiscuous biotin ligase. BioID2 enables more-selective targeting of fusion proteins, requires less biotin supplementation, and exhibits enhanced labeling of proximate ...

Manual correction of speech transcription can involve a selection from plausible transcriptions. Recent work has shown the feasibility of employing a mismatched crowd for speech transcription. However, it is yet to be established whether a mismatched worker has sufficiently fine-granular speech perception to choose among the phonetically proximate options that are likely to be generated from th...

The expectation that information and communication technologies lead users to transcend the constraints of physical separation has been expressed as the death of distance. Perceptions of distance – the psychological distance – significantly influence how individuals evaluate events and objects. As computer mediated communication technologies allow individuals to interact as easily with those wh...

This study presents the proximate and mineral composition of Peperomia pellucida L., an underexploited weed plant in Malaysia. Proximate analysis was performed using standard AOAC methods and mineral contents were determined using atomic absorption spectrometry. The results indicated Peperomia pellucida to be rich in crude protein, carbohydrate and total ash contents. The high amount of total a...

The phytochemical and proximate analyses of Psidium guajava leaves were determined using the methods of association of official analytical chemists (A.O.A.C). The quantitative analyses showed the concentrations (mg/100g) of the phytochemical constituents as flavonoids (0.40 ± 0.02), tannins (0.55 ± 0.01), glycosides (3.76 ± 0.08), alkaloids (0.04 ± 0.00), saponins (3.01 ± 0.01), phenols (0.28 ±...

Wild edible plants (WEPs) are the natural food source that can help to mitigate insecurity and improve starvation in low income countries including Ethiopia. Despite widespread use of WEPs Ethiopia, studies on nutritional contents Ethiopian limited. The objective current study is evaluate value (proximate, mineral, vitamin C contents) most consumed seven wild collected from Lasta District, Nort...

This study evaluated the effectiveness of dry Moringa oleifera leaf powder in the management of anaemia in adult albino rats. The proximate, mineral, vitamin and phytochemical composition of dry M. oleifera leaf powder were analysed. Twelve adult albino rats grouped into three were used. Cyclophosphamide was used to induce anaemia into them. The percentage proximate values were protein (26.28%)...

A major challenge of invasion biology lies in the development of a predictive understanding of invasion processes. Attempts to identify the proximate causes of invasion success or to predict rates of spread seldom emphasize behavioral characteristics. Recent experimental work, however, illustrates that insight into the proximate causes of animal invasions often hinges on a careful assessment of...