نتایج جستجو برای: protoplast cell

تعداد نتایج: 1684812  

2016
Mickael Malnoy Roberto Viola Min-Hee Jung Ok-Jae Koo Seokjoong Kim Jin-Soo Kim Riccardo Velasco Chidananda Nagamangala Kanchiswamy

The combined availability of whole genome sequences and genome editing tools is set to revolutionize the field of fruit biotechnology by enabling the introduction of targeted genetic changes with unprecedented control and accuracy, both to explore emergent phenotypes and to introduce new functionalities. Although plasmid-mediated delivery of genome editing components to plant cells is very effi...

Journal: :Biotechnology journal 2008
Mitra Mazarei Hani Al-Ahmad Mary R Rudis C Neal Stewart

Transient assay systems using protoplasts have been utilized in several plant species and are a powerful tool for rapid functional gene analysis and biochemical manipulations. A protoplast system has not been used in switchgrass (Panicum virgatum L.), even though it is a bioenergy crop that has received considerable attention. Here we report the first protocol to isolate large numbers of viable...

Journal: :Journal of bacteriology 1975
R S Rosenthal G D Shockman

Protoplasts of Streptococcus faecalis ATCC 9790 were produced with the aid of lysozyme, and the ability of these bodies to synthesize soluble, peptide cross-linked peptidoglycan (PG) fragments was examined. Lysozyme digests of PG isolated using gel filtration from the supernatant medium of protoplasts grown in the presence of [14C]acetate and L-[3H]lysine contained small amounts of PG having KD...

2014
Ingeborg Lang Stefan Sassmann Brigitte Schmidt George Komis

Plasmolysis is a typical response of plant cells exposed to hyperosmotic stress. The loss of turgor causes the violent detachment of the living protoplast from the cell wall. The plasmolytic process is mainly driven by the vacuole. Plasmolysis is reversible (deplasmolysis) and characteristic to living plant cells. Obviously, dramatic structural changes are required to fulfill a plasmolytic cycl...

Journal: :Plant physiology 1989
S Gilroy W A Hughes A J Trewavas

Assessment of the regulation of plant metabolism by the calcium ion requires a knowledge of its intracellular levels and dynamics. Technical problems have prevented direct measurement of the concentration of intracellular Ca(2+) in plant cells in all but a few cases. In this study we show that electropermeabilized protoplasts of Daucus carota and Hordeum vulgare took up the Ca(2+) indicating fl...

2013
HaiKuan Wang Yao Sun Chong Chen Zhe Sun YanChao Zhou FaDi Shen HePing Zhang YuJie Dai

Many species of lactic acid bacteria have the ability to inhibit the growth of spoilage fungi in food and feed products. Genome shuffling is a powerful process that could efficiently improve desirable industrial phenotypes of microbial strains through recursive protoplast fusion. In our study, the antifungal activity of Lactobacillus plantarum IMAU10014 was improved by genome shuffling. The sta...

2013
Peter M. Letcher Salvador Lopez Robert Schmieder Philip A. Lee Craig Behnke Martha J. Powell Robert C. McBride

Mass culture of algae for the production of biofuels is a developing technology designed to offset the depletion of fossil fuel reserves. However, large scale culture of algae in open ponds can be challenging because of incidences of infestation with algal parasites. Without knowledge of the identity of the specific parasite and how to control these pests, algal-based biofuel production will be...

Journal: :Methods in molecular biology 2007
Ping He Libo Shan Jen Sheen

The use of plant protoplast transient expression system has facilitated the discovery and dissection of many signal transduction pathways in response to hormones, metabolites, and stresses. Recently, Arabidopsis protoplasts also have been used successfully to study plant innate immune responses triggered by pathogen-derived elicitors. Here, we describe the detailed protocols for studying innate...

2012
Emma Jane Smith Rebecca M Corrigan Tetje van der Sluis Angelika Gründling Pietro Speziale Joan A Geoghegan Timothy J Foster

The Sbi protein of Staphylococcus aureus comprises two IgG-binding domains similar to those of protein A and a region that triggers the activation of complement C3. Sbi is expressed on the cell surface but its C-terminal domain lacks motifs associated with wall or membrane anchoring of proteins in Gram-positive bacteria. Cell-associated Sbi fractionates with the cytoplasmic membrane and is not ...

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