■ ■ CSH130 C18 is QC tested with a tryptic digest of cytochrome c IN T RODUC T ION Peptide mapping of a biopharmaceutical, when employed for quality control, has traditionally involved detection by UV absorbance. However, to characterize the species in a peptide map, LC separations often must be coupled with ESI-MS. Mobile phases containing trifluoroacetic acid (TFA) have been almost exclusivel...

People living with visual impairments face important challenges related to orientation and mobility which might even prevent them from travelling autonomously. Indeed, it is difficult to navigate in an unknown space in the absence of vision or to acquire knowledge about spaces before travelling. Historically, raised-line paper maps with braille text have been used to teach geography to visually...

acute dyspnea is a common cause of hospitalization in emergency departments (ed).distinguishing the cardiac causes of acute dyspnea from pulmonary ones is a major challenge for responsible physicians in eds. this study compares the characteristics of bedside ultrasonography with serum level of blood natriuretic peptide (bnp) in this regard. methods: this diagnostic accuracy study compares bedsi...

background hypervariability of hcv proteins is an important obstacle to design an efficient vaccine for hcv infection. multi-epitope vaccines containing conserved epitopes of the virus could be a promising approach for protection against hcv. objectives cellular and humoral immune responses against multi-epitope dna and peptide vaccines were evaluated in balb/c mice. materials and methods in th...

The aim of this study was to use the microtitre alamarBlue assay to investigate and compare the antimycobacterial potential of the lantibiotics nisin and lacticin 3147 against a representative cohort of clinically significant mycobacteria, i.e. Mycobacterium tuberculosis H37Ra, Mycobacterium avium subsp. paratuberculosis (MAP) ATCC 19698 and Mycobacterium kansasii CIT11/06. Lacticin 3147 displa...

Mitogen-activated protein (MAP) kinases have been demonstrated to have a role in meiosis but their involvement in mitotic events is less clear. Using a peptide antibody raised against the tobacco MAP kinase p43(Ntf6) and extracts from synchronized tobacco cell suspension cultures, we show that this kinase is activated specifically during mitosis. Entry into mitosis appears to be necessary for t...

Ivan C. Rankenburg and Veit Elser Abstract We demonstrate a new algorithm for finding protein conformations that minimize a non-bonded energy function. The new algorithm, called the difference map, seeks to find an atomic configuration that is simultaneously in two constraint spaces. The first constraint space is the space of atomic configurations that have a valid peptide geometry, while the s...

MAP kinases phosphorylate specific groups of substrate proteins. Here we show that the amino acid sequence FXFP is an evolutionarily conserved docking site that mediates ERK MAP kinase binding to substrates in multiple protein families. FXFP and the D box, a different docking site, form a modular recognition system, as they can function independently or in combination. FXFP is specific for ERK,...

Accumulating evidence suggests that the insect and mammalian innate immune response is mediated by homologous regulatory components. Proinflammatory cytokines and bacterial lipopolysaccharide stimulate mammalian immunity by activating transcription factors such as NF-kappaB and AP-1. One of the responses evoked by these stimuli is the initiation of a kinase cascade that leads to the phosphoryla...