introduction: in this study in vitro maturation, resumption of meiosis and development of immature mouse oocytes in the presence of cysteamine was evaluated. meterial and methods: immature oocytes recovered from nmri mouse strain (4-6 weeks) were categorized in three experimental and one control group. the control included 345 immature oocytes which were matured in mem medium contain 5% fcs. in...

we examined the demographic characteristics of couples, ovarian response and sperm quality to determine the predictors of clinical fertilization in ivf cycles. a descriptive and analytic study was conducted using data and biologic specimens obtained fatemeh-alzahra of babol infertility center from 2004 to 2005. only data from 315 women who had medical indication for conventional ivf treatment w...

Polycystic ovary syndrome (PCOS) is typically characterized by a polycystic ovarian morphology, hyperandrogenism, ovulatory dysfunction, and infertility. Furthermore, PCOS patients undergoing stimulation have more oocytes; however, the poor quality of oocytes leads to lower fertilization implantation rates, decreased pregnancy increased miscarriage rates. The complex molecular mechanisms underl...

Abstract Study question Can we visualise model oocytes and embryos during embryo development using OCT non-invasively? Summary answer This approach, new to embryology, OCT, allows visualising cells in a 3D perspective, providing more detailed information than standard microscopy from the oocyte or embryo. What is known already Time-lapse well-established technique human IVF, but it limited its ...

The roles of calmodulin-binding sites in the regulation by Ca2+, inositol 1,4,5-trisphosphate (InsP3) and GTP-binding regulatory proteins (G-proteins) of the Drosophila melanogaster TRPL (transient-receptor-potential-like) non-specific Ca2+ channel were investigated. Wild-type TRPL protein and two mutant forms, TRPL (W713G) and TRPL (W814G), in which a key tryptophan residue in each of the two ...

objective: retinoids are recognized as important regulators of cell differention and tissue function. previous studies, performed both in vivo and in vitro, indicate that retinoids influence several reproductive events. in this study, we investigated the effect of all-trans retinoic acid (t-ra) on maturation and fertilization rate of immature oocytes (germinal vesicle). materials and methods: g...

Abstract Study question Do the oocytes retrieved after luteal phase ovarian stimulation (OS) have same survival rate as those follicular (OS)? Summary answer Oocyte vitrification is similar in a OS compared to phase. What known already Oocytes recently captured scientific interest dual gaining popularity. obtained added shorten time pregnancy per intention treat poor responders. Embryos with th...

BACKGROUND The ovulatory gonadotropin surge increases synthesis of prostaglandin E2 (PGE2) by the periovulatory follicle. PGE2 actions on granulosa cells are essential for successful ovulation. The aim of the present study is to determine if PGE2 also acts directly at the oocyte to regulate periovulatory events. METHODS Oocytes were obtained from monkeys and mice after ovarian follicular stim...

Although differences in the quality of oocytes derived from young gilts and adult sows are well documented, evidence concerning gametes of pre-pubertal and cycling gilts is scarce and inconsistent. The aim of this work was to establish whether sexual maturity of gilts affects the quality of their oocytes with the use of the brilliant cresyl blue (BCB) test, oocyte diameter and apoptosis. Ovaria...

Amphibian oocytes were incubated in vitro in the presence of [H]uridine, and autoradiographs were made of nucleoli isolated from these oocytes and of sections of oocytes. After incubations of a h or less the nucleoli of oocytes larger than o-6 mm diameter are asymmetrically labelled. With longer incubations nucleoli from oocytes of o-6 to r i mm diameter become more uniformly labelled. Those of...