Three different types of biomass sourced from forestry waste (eucalyptus residues), agricultural (wheat straw), and energy crop (miscanthus) were used as starting materials to produce hemicellulosic sugars, furans (furfural hydroxymethylfurfural), oligosaccharides. A two-step hybrid process was implemented; first autohydrolysed without any additive extract hemicelluloses dissolve it in water. T...

A great number of naturally occurring oligosaccharides and oligosaccharide esters have been isolated from traditional Chinese medicinal plants, which are used widely in Asia and show prominent curative effects in the prevention and treatment of kinds of diseases. Numerous in vitro and in vivo experiments have revealed that oligosaccharides and their esters exhibited various activities, includin...

The use of novel mass spectrometric techniques, off-line coupled to chromatographic techniques is described in order to obtain molecular mass information of compounds present in complex mixtures of oligosaccharides. Such information could be quite useful in studies where pure and well-defined enzymes are used to study the chemical fine structure of polysaccharides. Post source decay fragmentati...

The O-linked oligosaccharides attached to human erythrocyte glycophorins were extensively characterized. In addition to the previously described disialylated tetrasaccharide, NeuNAc alpha 2----3Gal beta 1----3 (Neu-NAc alpha 2----6)GalNAcOH and monosialylated trisaccharide, NeuNAc alpha 2----3Gal beta 1----3GalNAcOH, novel trisialylated oligosaccharides were isolated. Methylation analysis, fast...

INTRODUCTION Glycoforms and Protein Sialylation Glycoproteins are proteins with a carbohydrate attached to the polypeptide backbones through one or more glycosylation sites. Oligosaccharides can be linked to a protein through a serine or a threonine as O-linked glycans, or through an asparagine as N-linked glycans. Glycoprotein glycoforms contain identical polypeptide backbones and differ from ...

The tosyl functional group is commonly used in carbohydrate chemistry as a nucleofuge. Tosylation of the primary hydroxyls carbohydrates are generally performed after orthogonal protection/deprotection reactions. However, it can be done regioselectively from unprotected sugars. Several examples have been described literature starting free monosaccharides. Yields good but may vary according to n...

Pectin oligosaccharides, which can be obtained from fruit wastes, have proven their potential as plant immune-system elicitors. Although the precise size of active species is still under investigation, medium oligosaccharides been reported most active. Three defined oligogalacturonic acid (OGAs) mixtures were produced commercial pectin, orange peel and apple pomace residues. The methodology dev...

The aldehyde forms of saccharides are the major reactants in Maillard reaction sugars with free amine groups proteins. ratios aqueous solutions crucial to rate determining reaction. disaccharides and oligosaccharides have been barely analyzed experimentally or computationally. Here, we tested feasibility quantum chemical calculation various levels solution models predict comprising d-glucose. r...

Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrosp...

Following the rapid enzymatic transfer of an oligosaccharide (GlcNAc2Man9Glc3) from a lipid carrier to endogenous protein acceptors in membrane preparations from NIL fibroblasts, the transferred oligosaccharide chain undergoes processing. Protein-bound oligosaccharides, released from the polypeptide backbone by treatment with endo-beta-N-acetylglucosaminidase H, were analyzed by gel filtration ...