Halobacterium volcanii mutants that are resistant to the dihydrofolate reductase inhibitor trimethoprim contain DNA sequence amplifications. This paper describes the cloning and nucleic acid sequencing of the amplified DNA sequence of the H. volcanii mutant WR215. This sequence contains an open reading frame that codes for an amino acid sequence that is homologous to the amino acid sequences of...

The ongoing pandemic and increasing frequency of infectious disease outbreaks due to climate change, urbanization, global human migration have put great focus on nucleic acid amplification-based diagnostic technologies (NAAT). These methods can provide gold standard accuracy sensitivity, their widespread availability at the point-of-care is crucial for managing spread pathogens in animal popula...

BACKGROUND Genotyping methods that reveal single-nucleotide differences are useful for a wide range of applications. We used digestion of 3-way DNA junctions in a novel technology, OneCutEventAmplificatioN (OCEAN) that allows sequence-specific signal generation and amplification. We combined OCEAN with peptide-nucleic-acid (PNA)-based variant enrichment to detect and simultaneously genotype v-K...

We report on the development of an on-chip RPA (recombinase polymerase amplification) with simultaneous multiplex isothermal amplification and detection on a solid surface. The isothermal RPA was applied to amplify specific target sequences from the pathogens Neisseria gonorrhoeae, Salmonella enterica and methicillin-resistant Staphylococcus aureus (MRSA) using genomic DNA. Additionally, a posi...

In the 19 years since the first descriptions of the PCR (1 ), nucleic acid amplification methods have made the transition from research to clinical laboratories. Molecular diagnostics are now firmly established as part of laboratory medicine, with applications in genetics, oncology, pharmacology, and infectious disease. Routine diagnostic applications of these methods have been made possible by...

objective: toxoplasmosis is a worldwide disease, for which different detection methods have been used. the nucleic acid sequence-based amplification (nasba) method is shown to be highly efficient for diagnosis of live microorganisms. the present research evaluates the molecular isothermal method of nasba to identify live toxoplasma gondii (t. gondii) in rat. methods: tachyzoites of t. gondii w...

I hereby certify that this material, which I now submit for assessment on the programme of study leading to the award of B.Sc. in Applied Physics is entirely my own work, does not to the best of my knowledge breach any law of copyright, and has not been taken from the work of others save and to the extent that such work has been cited and acknowledged within the text of my work. Abstract The ar...

Development of rapid amplification assays for the detection and identification of biological threat agents has become a focus of diagnostic efforts in recent years. The use of real-time PCR assays as diagnostic tools depends upon two critical processes. First, nucleic acid purification must provide template that is both amplifiable and free of PCR inhibitors. Second, the assays themselves must ...

Accurate and timely detection of infectious pathogens is urgently needed for disease treatment control possible outbreaks worldwide. Conventional methods pathogen are usually time-consuming labor-intensive. Novel strategies the identification pathogenic nucleic acids necessary practical application. The advent microfluidic technology devices has offered advanced miniaturized tools to rapidly sc...