Influenza C virus (strain C/Johannesburg/1/66) was grown, harvested, purified and used as source for the enzyme O-acetylesterase (N-acyl-O-acetylneuraminate O-acetylhydrolase; EC 3.1.1.53). This activity was studied and characterized with regard to some new substrates. The pH optimum of the enzyme is around 7.6, its stability at different pH values shows a result similar to that of the pH optim...

Two types of microemulsion reaction systems: simple and mixed reverse micelles have been investigated as potential bioreactors for a transglycosylation reaction catalyzed by three microbial b-galactosidases: fungal Aspergillus oryzae, yeast Kluyveromyces marxianus and bacterial Escherichia coli b-galactosidase. Several issues such as: the effect of the degree of hydration on the total enzyme ac...

The effect of p-nitrophenyl beta-D-xyloside, an inhibitor of proteoglycan biosynthesis, on the growth of chick embryos was studied by injection of the single dose of 1.0 mg/egg into fertile eggs on day 3. Embryos examined on day 10 had systemic edema, and were increased not only in wet weight (142% of the non-treated embryos) but also in dry weight (125%). No skeletal malformations were observe...

Two cellulase cDNAs, celB29 and celB2, were isolated from a cDNA library derived from mRNA extracted from the anaerobic fungus, Orpinomyces joyonii strain SG4. The nucleotide sequences of celB2 and celB29 and the primary structures of the proteins encoded by these cDNAs were determined. The larger celB29 cDNA was 1966bp long and encoded a 477 amino acid polypeptide with a molecular weight of 54...

The ever-evolving research in the field of antitubercular agents has led to identification several new potential drug classes. Among them, 5-phenyl-furan-2-carboxylic acids have emerged as innovative therapeutics, targeting iron acquisition mycobacterial species. In our efforts characterize molecular interactions between these compounds and their protein target (MbtI from M. tuberculosis) by me...

Objective(s): The novel 1,5-diaryl-1,4-pentadien-3-one derivatives were studied for analgesic, anti-inflammatory and anticancer potential to establish their role in pain, inflammatory disorders and cancer.Materials and Methods: Two 1,5- diaryl-1,4-pentadien-3-one derivatives: (1E,4E)- 5-(4-fluoro phenyl)-1-(4-methoxyphenyl)- 2-methylpenta-1,4-dien-3-one (A2K2A17) and  (1E,4E)-5-(4-nitrophenyl)-...