Bone marrow CD34(+) cell apoptosis (annexin V), proliferation (Ki-67), and Bcl-2-related protein expression was evaluated by flow cytometry in 102 patients with myelodysplastic syndrome (MDS) and acute myeloid leukemia secondary to MDS (MDS-AML) and in 30 normal donors (NBM). Apoptosis was significantly increased in refractory anemia (RA)/RA with ringed sideroblasts (RARS) (56.9% [20.4%-93.6%])...

Introduction Anterior cruciate ligament (ACL) tear is a common knee injury, and is a risk factor of post-traumatic osteoarthritis (OA) [1]. The disease is frequently associated with bone marrow edema-like (BMEL) lesions which exhibit as an area of high signal intensity in T2-weighted, fat-saturated fast spin echo MR images. BMEL is also commonly seen in OA and has been associated with disease p...

In this research study an approach of creating dictionary of critical terms is used to assess the bug severity as severe and non severe. It is found that using different approaches of feature selection and classifier the pattern of accuracy and precision is approximately same. However Chi square test and KNN classifier give the maximum performance of precision and accuracy for the all four comp...

Abstract Aims High levels of morbidity and mortality are associated with small bowel obstruction (SBO), a common general surgical emergency. Assessment malnutrition introduction early nutritional interventions paramount importance in the management algorithm these patients. We audited our practice managing acute at busy district hospital comparing against National Audit Small Bowel Obstruction ...

Some animal models have been used to study Alzheimer's disease (AD). AD is an irreversible progressive neurodegenerative disease and the most common cause of dementia. Animal studies have shown that there is a relation between decrease in cholinergic functions in the nucleus basalis of Meynert (NBM) and loss of learning capability and memory. The aim of this study was to investigate the effect ...

BACKGROUND The anti-cancer activities of intravenous anesthetic drug propofol have been demonstrated in various types of cancers but not in chronic myeloid leukemia (CML). METHODS We systematically examined the effect of propofol and its combination with BCR-ABL tyrosine kinase inhibitors (TKIs) in CML cell lines, patient progenitor cells and mouse xenograft model. We analyzed propofol's unde...

Macrophage inflammatory protein-1a (MIP-1a) can stimulate growth inhibitory and potent chemotactic functions in hematopoietic cells. To investigate whether the action of MIP-1a may be regulated at the cellular receptor level, we studied the expression and modulation of MIP-1a receptors on CD341 cells isolated from normal bone marrow (NBM), umbilical cord blood (CB), and leukapheresis products (...

In this video, we demonstrate the preparation of E18 cortical rat neurons. E18 cortical rat neurons are obtained from E18 fetal rat cortex previously dissected and prepared. The E18 cortex is, upon dissection, immediately dissociated into individual neurons. It is possible to store E18 cortex in Hibernate E buffer containing B27 at 4 degrees C for up to a week before the dissociation is perform...