Amongst a large number of Populus species, micropropagation of P. deltoides has not been possible. The authors now report a reproducible protocol for mass production of cloned plants of 2 important clones of P. deltoides, viz., G3 and G48 through induced shoot bud differentiation in the in vitro cultured segments of leaf, stem and root of adult tree origin. Establishment of experimental plants ...

Subterranean clover inoculated or not with the arbuscular mycorrhizal (AM) fungus Glomus intraradices was grown on soil containing six levels of 238U in the range 0-87 mg kg(-1). Increasing U concentration in soil enhanced the U concentration in roots and shoots of both mycorrhizal and nonmycorrhizal plants but had no significant effects on plant dry matter production or root AM colonization. M...

This study, using the method of direct collection feces, investigated captive giant pandas daily intakes and apparent digestibility coefficients of diet dominantly comprised of Phyllostachys vivax bamboo shoots. The results showed that the captive giant pandas daily ingested ~2818 g (dry mass) of bamboo shoots (corresponding to ~42 kg of fresh bamboo shoots with shells). Based on this daily int...

The effect of salinity on the activity of glutamine synthetase (GS EC 6.3.1.2.) and glutamate dehydrogenase (NAD(P)H-GDH EC 1.4.1.2-4) in shoots and roots of triticale seedlings was investigated. Stressed plants were cultured hydroponically on the 100 mM NaCl-enriched medium for 7 days and the physiological responses were measured. Apart from changes in morphology of plants, alterations in GS a...

A highly efficient protocol for in vitro propagation of Rauwolfia serpentina L. was developed that consisted of callus induction, multiple shoot regeneration and rooting stages. Effect of different plant growth regulators, exclusively or in combination on callus induction, multiple shoot formation and root induction, were observed. Maximum callusing (93.65%) were obtained in leaf and stem expla...

Tissue culture is considered as an alternative tool for garlic breeding as traditional breeding is not possible due to its sterility. A study was carried out to develop a protocol of regeneration in Bangladesh local garlic. Shoot and root meristems of garlic cv. Bangladesh local were cultured on Murashige and Skoog (MS) medium containing various growth regulator combinations. Regeneration from ...

An efficient procedure for in vitro shoot production of the medicinal plant sundew (Drosera rotundifolia L.) was developed. Of three cytokinins tested, zeatin (ZEA) at a concentration of 2 μM resulted in the formation of large numbers of adventitious shoots on leaf explants. A larger size of the small shoots was achieved by combining a 2-weeks preculture with ZEA (step 1: shoot induction) with ...

Axenic shoot culture of Mentha arvensis was established from young shoots of plants cultivated in a field. In the shoots cultured on Murashige-Skoog solid and liquid media for 3-4 weeks, pulegone was the main constituent in both cases, and the content of pulegone in liquid medium was much more than that on solid medium. The shoots cultured in the dark for 3 weeks produced only pulegone. However...

The present study was undertaken to investigate the possibility for developing Efficient Plant regeneration protocol in Vigno mungo L. For this, the effect of Cytokinens on in vitro regeneration from Cotyledonary explants was studied in Black gram (Vigno mungo L.) Hepper Var Vamban-1. The cytokinins used were 6-benzylaminopurine (BAP), Thidiazuron (TDZ), and kinetin (Kn). The regenerated shoots...

The branch construction of trees is based on phytomers, repetitive subunits defined as node + leaf + axillary meristem + internode. The rate at which phytomers are added to a shoot is termed the phyllochron, which is determined by genetics, endogenous regulation and environmental conditions. The phyllochron is fundamental to understanding the growth of plants. Most phyllochron studies on woody ...