prohibitins are chaperone proteins highly conserved among eukaryotes. many different functions have been attributed to prohibitins depending on their tissue expression and sub-cellular localization. prohibitins play a major role in mitochondrial physiology, regulating the stability and the processing of both respiratory chain complex subunits and opa1, a mitochondrial fusion regulating protein....

Objective: The objective of the present study was to evaluate the cytotoxic potentials of Evolvulus alsinoides in human hepatoma HepG2 cells. Materials and Methods: HepG2 cells were treated with methanolic extract of E. alsinoides at 20, 40 and 80 µg/ml for 24 hr and cytotoxic effect was analyzed by MTT assay. The apoptosis rate was investigated by Hoechst 33342 and annexin V/propidium iodide s...

Ema19 is an integral endoplasmic reticulum (ER) protein with relevance for mitochondrial biogenesis. This yeast, representative of the TMEM97/sigma 2 receptor family, serves as quality control factor which supports degradation nonimported proteins. study again demonstrates important role ER in targeting.

1. Recently we reported decreased activities of two mitochondrial marker enzymes (citrate synthase and cytochrome c oxidase) in skeletal muscle from a rat model of critical illness (zymosan injection). In the present study we investigated (i) whether these decreases in enzyme activity reflect a reduction in mitochondrial content and (ii) whether this potential reduction in mitochondrial content...

Mitochondrial protein import depends on heterooligomeric translocases in the outer and inner membranes. Using substrates consisting of various lengths N-terminal part mitochondrial dihydrolipoamide dehydrogenase (LDH) fused to dihydrofolate reductase we present an vivo analysis showing that Trypanosoma brucei at least 96 aa mature LDH are required efficiently produce intermediate spans both tra...

Hydrazide-containing bifunctional nanospheres were covalently coupled on the surface with IgG, avidin, and biotin, to generate novel fluorescent-magnetic-biotargeting trifunctional nanospheres, which can be used in a number of biomedical applications, including visual sorting and manipulation of apoptotic cells as demonstrated here.