Surface treatment of biological tissue sections improves detection of peptides and proteins for mass spectrometry imaging. However, liquid surface treatments can result in diffusion of surface analytes and fragile tissue sections can be easily damaged by typical washing solvents. Here, we present a new surface washing procedure for mass spectrometry imaging. This procedure uses solvent wetted f...

This paper studies the effect of photosensitizer (acetone, H(2)O(2), surfactant and pigment) on photodegradation of diclofenac (DCF) under simulated sunlight. The results demonstrate that degradation pathways proceed via pseudo first-order kinetics in all cases. The photodegradation rate was found to increase with increasing acetone and H(2)O(2). Surfactant and pigment inhibited the photodegrad...

The aim of the present investigation was to biotransform the anti-inflammatory compound meloxicam by enzymes present in whole cells of five actinomycete cultures to produce novel bioactive derivatives. Among the actinomycetes screened, Streptomyces griseus NCIM 2622 was found to possess the enzyme system(s) that oxidize meloxicam into two metabolites whereas that present in S. griseus NCIM 2623...

Studies are described on the phase I and II metabolism and the toxicological analysis of the piperazine-derived designer drug 1-(3-trifluoromethylphenyl)piperazine (TFMPP) in rat urine using gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS). The identified metabolites indicated that TFMPP was extensively metabolized, mainly by hydroxylation of the ...

Bioanalysis is sub-discipline of analytical chemistry covering the quantitative measurement of drugs and their metabolites in biological systems. Whereas Liquid chromatography mass spectrometry is a technique which is widely used for the quantification of drug from biological fluid. This article reviews the most recent advances in sample preparation, separation analysis and different type of ca...

A peptide toxin was isolated from a waterbloom of Microcystis aeruginosa from Lake Frøylandsvatn in Norway. The isolation procedure included liquid and solid phase extraction and reversed phase high performance liquid chromatography. Amino acid analysis yielded D-glutamic acid, D-erythro-beta-methylaspartic acid and D-alanine in equimolar and L-arginine in twofold molar ratios. The presence of ...