Somatic embryogenesis is an efficient micro-propagation technique, which can lead to mass multiplication of tea with a tap root. Hence, the development viable somatic and protocol through liquid culture conditions needed increase efficiency protocol. Cotyledon-derived embryos, leaf stem callus TRI 5001 cultivar, were tested different MS media. A significantly higher cotyledon-derived embryo rat...

background: sustainable and commercial production of taxol as an anti cancer drug is a critical point to its clinical application. nowadays, hazel because of rapid growth and wide range distribution is considered as an alternative source of taxol. objective: to increase taxol production the cdna encoding 3-hydroxy-3-methylglutaryl-coa reductase (hmgr) from iranian hazel (genebank accession numb...

Extracts from raw materials from different plant parts, tissue-cultured plants, and callus cultures of Oroxylum indicum were analyzed for in vitro antioxidant activities determined by DPPH radical scavenging assay and evaluated for phytochemical profiles by TLC and LC-MS methods. The results were analyzed by principal component analysis (PCA) to evaluate the similarity. Stalk, pedicel, flower, ...

In vitro morphogenesis is greatly influenced by plant growth regulators (PGRs). The effect of different plant growth regulators was assessed on callus induction, in vitro shoot regeneration and hairy root formation in tomato. The maximum callus induction frequency was recorded culturing hypocotyls, while in vitro shoot regeneration frequency was significantly higher when leaf discs were used as...

Various in vitro grown tissues (non-regenerative callus, regenerative callus and microshoot derived leaves) of Solanum nigrum L. were cultured under salinity stress (0-150 mM NaCl) for enhanced production of solasodine, a steroidal alkaloid and an alternative to diosgenin, which is used as a precursor for the commercial production of steroidal drugs. The role of plant growth regulators and vari...

Forsythia spp. are perennial woody plants which are one of the most extensively used medicinal sources of Chinese medicines and functional diets owing to their lignan contents. Lignans have received widespread attention as leading compounds in the development of antitumor drugs and healthy diets for reducing the risks of lifestyle-related diseases. However, the molecular basis of Forsythia has ...

This study investigated the effects of different PGRs on in vitro plant regeneration Anthurium scherzerianum Schott and acclimatization ex stage. Depending objective each experiment, shoots were induced from callus cultured a modified Murashige Skoog (MS) medium containing cytokinins auxins. Results showed that 90% leaf explants 48% petiole produced callus. A half-strength MS supplemented with ...

Gymnema sylvestre (R.Br.) is an important diabetic medicinal plant which yields pharmaceutically active compounds called gymnemic acid (GA). The present study describes callus induction and the subsequent batch culture optimization and GA quantification determined by linearity, precision, accuracy, and recovery. Best callus induction of GA was noticed in MS medium combined with 2,4-D (1.5 mg/L)...

Hypericum triquetrifolium Turra. is a valuable medicinal plant owing to source of many bioactive compounds. Hypericin is one of the significant components among these compounds. The aim of this study was to develope an efficient method allowing to improve hypericin production from calli and cell suspension cultures of Hypericum triquetrifolium Turra. Callus formation was obtained from axenic le...

Digitalis davisiana, commonly called Alanya foxglove, from Turkey, is an important medicinal herb as the main source of cardiac glycosides, cardenolides, anthraquinones, etc. It is also known in the Indian Medicine for treatment of wounds and burns. It has ornamental value as well. Overexploitation of D. davisiana has led this species to be declared protected, and thereby encouraged various met...