Autophagic sequestration of endogenous lactate dehydrogenase or electroinjected [3H]raffinose in isolated rat hepatocytes was strongly suppressed by the Ca2+ chelator EGTA, unless the cells had previously been electroloaded in the presence of high concentrations of Ca2+ (1.2 mM). The extracellular Ca2+ chelator bis-(o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid (BAPTA) and the intracellular...

New fluorescent ionophores containing the 1-naphthylacetanilide moiety based on oligo-ethylene oxide (1(4) and 1(5)) have been synthesized, and their photochemical behaviors have been studied. In the absence of a metal ion, the 1-naphthylacetanilide moiety showed a weak fluorescence emission (fluorescence "off state"). However, complexation with Ca(2+) induced a large enhancement effect on the ...

Coccidiosis is likely the most costly disease threat to global poultry production and prevention of coccidiosis is essential in order to maintain intestinal integrity and the efficient absorption of nutrients in the chicken. Narasin is an ionophore anticoccidial. Ionophores transport cations such as sodium across the pellicle membrane of sporozoites of coccidia, resulting in a lethal osmotic im...

Two taste preference studies were conducted using six Holstein heifers in each experiment to determine preferences for no ionophore, lasalocid, or monensin in the diet. In Exp. 1, individually penned (approx. 5 mo old; 220 +/- 14 kg BW) heifers were fed a basal total mixed ration containing 46% corn silage, 46% grass haylage, and 8% soybean meal (DM basis). There were five treatments (mg/kg BW(...