To define more precisely the inoculation methods to be used in the oxacillin screen test for Staphylococcus aureus, we tested agar screen plates prepared in house with 6 microg of oxacillin/ml and 4% NaCl using the four different inoculation methods that would most likely be used by clinical laboratories. The organisms selected for testing were 19 heteroresistant mecA-producing strains and 41 n...

a building contractor by occupation. The back of the volunteer's shoulder was selected for inoculation, as it was under the clothes and covered by the shirt. The skin was sterilised with tincture of iodine and washed with absolute alcohol. A hypodermic Record syringe was sterilised in olive oil at 140?C, cooled, and then filled with 1 c.c. of the culture of Leishmania tropica from the water of ...

Determination of the drug susceptibility of Mycobacterium tuberculosis by conventional methods using an agar-based medium may take 3 weeks or more to complete. On the other hand, results on positive cultures are generally available in 4 to 7 days with the radiometric (BACTEC, Johnston Laboratories, Towson, Md.) procedure. One disadvantage to the latter is the requirement to determine the quanti...

An isolate of the filamentous cyanobacterium Microcoleus sp. was obtained from black mangrove aerial root (pneumatophore) and inoculated onto young mangrove seedlings to evaluate N2-fixation and rootcolonization capacities of the bacterium under in vitro conditions in closed-system experiments. N2 fixation (acetylene reduction) gradually increased with time and reached its peak 5 days after ino...

The objective was to evaluate the inoculation, co-inoculation with bacteria B. japonicum and A. brasilense in combination application of N doses on agronomic characteristics soybean. design used randomized blocks a 4x4 factorial scheme, four types inoculation (absence bacteria, brasilense) (0 % = 0; 2 4.5; 4 9 8 18 kg ha-1), replications. increasing above 2% promoted reduction number nodules fo...

The effects of three Arbuscular Mycorrhizal Fungi (AMF) species (Glomus versiforme, Claroideoglomus etunicatum and Rhizophagus intraradices) were studied on the growth parameters and nutrient uptake of three apple rootstocks (M.9, M.7 and MM.106). The soil medium contained a high level of calcium carbonate (38.65%). The results showed that AMF inoculation could increase almost all growth parame...

Vibrio fetus is usually considered an organism difficult to grow on primary isolation. Even after adaptation to laboratory media growth remains scanty, and frequent transfers are required. However, we have found that excellent growth can be obtained on thioglycolate medium. (Brewer modified), which contains per liter: trypticase, 17.0 g; phytone, 3.0 g; glucose, 6.0 g; sodium chloride, 2.5 g; s...

the combined effects of inoculation level (4 or 8-fold compared to standard inoculation) and sequence (standard inoculation before fermentation and 3-fold inoculation at the end of fermentation=1+3, two-fold inoculation before fermentation and the same at the end of fermentation=2+2, 2+6, 4-fold before fermentation=4, 4+4, and 8) of culture inoculum containing probiotics on biochemical and micr...

Strain Hhs.015(T) (Saccharothrix yanglingensis sp. nov.), an antagonistic endophytic Saccharothrix actinomycete isolated from roots of cucumber plants, exhibited a broad antimicrobial spectrum in vitro and was active as a biocontrol against plant diseases in field trials. The SSY medium was used for production of antimicrobial metabolites by strain Hhs.015(T). However, this medium is too expens...

BACKGROUND In this study the concentration of lysozyme in blood plasma of Microtus agrestis, Clethrinomys glareolus, Apodemus sylvaticus, BK rats and outbred white mice before and after infection with culture forms of Trypanosoma microti, T, evotomys, T. grosi, T. lewisi and T. musculi respectively was measured. METHODS Blood samples of rodents, Microtus agrestis, Clethrionomys glareolus, Apo...