separation of some biogenic amines via rp-hplc using mixed micellar mobile phase was investigated. the compounds were derivatized before hand by dansyl chloride as a chromophoric reagent. appropriate conditions for separation, were determined by studying factors such as temperature, type and percentage of organic modifier, concentration of surfactants (sds and brij-35) and the ph of the mobile ...

A newly developed single quad mass spectrometry (MS) detector was coupled to a ultra-high performance liquid chromatography (UPLC) system and implemented in the routine quality control (QC) and impurity analysis of four therapeutic peptides, namely bleomycin sulfate, tyrothricin, vancomycin HCl and bacitracin, which were selected given their multi-component drug nature and their closely structu...

We have developed a simplified and improved high performance liquid chromatography (HPLC) method for the detection and quantitation of tissue lipid using a new laser light-scattering detector (Varex model ELSD II). This detector has a limit of sensitivity of 50 ng for neutral lipid and 200 ng for most phospholipids with excellent reproducibility. By coupling the ELSD II with a ternary gradient ...

A tadalafil analogue was detected in a dietary supplement marketed for tonic effect, along with hydroxyhomosildenafil and aminotadalafil. The tadalafil analogue was isolated by preparative thin layer chromatography (TLC) and its structure was elucidated using high-performance liquid chromatography (HPLC), liquid chromatography electrospray ionization-mass spectrometry (LC-ESI-MS), Fourier trans...

high-performance liquid chromatography (hplc) methods employing ultraviolet (uv) detector are not sufficiently sensitive to measure the low plasma concentrations following single oral dose of imipramine. therefore, in the present study a simple, rapid and yet sensitive hplc method with uv detection was developed and validated for quantitation of imipramine in human plasma samples. an efficient ...

A high-speed counter-current chromatography (HSCCC) method for preparative separation and purification of five hydroxyanthraquinones and cinnamic acid from the Chinese medicinal herb Rheum officinale Baill. was developed by using pH-gradient elution. The purities of rhein, emodin, aloe-emodin, chrysophanol, physcion and cinnamic acid were all over 98%, as determined by high performance liquid c...

A neurophysiological detector (NPD) is a hybridization of olfactory system neurons of the fish crucian carp, Carassius carassius L., with a computerized electronic device connected to a high-performance liquid chromatograph (HPLC). This system makes it possible to measure neurophysiological activities in the olfactory system of C. carassius L. after exposure of this fish to alarm pheromones. Th...

A sensitive (0.01-1 nmol) method has been developed for the analysis of polyamines in higher plant extracts based on high performance liquid chromatography (HPLC) of their benzoyl derivatives (Redmond, Tseng 1979 J Chromatogr 170: 479-481). Putrescine, cadaverine, agmatine, spermidine, spermine, and the less common polyamines nor-spermidine and homospermidine can be completely resolved by rever...