The fully dissociated hexon polypeptide of adenovirus type 2 has a molecular weight of 129,000 (+/-10,000) daltons as determined by high-speed sedimentation equilibrium in 6 m guanidine.

The kinetics of actin unfolding induced by guanidine hydrochloride has been studied. On the basis of obtained experimental data a new kinetic pathway of actin unfolding was proposed. We have shown that the transition from native to inactivated actin induced by guanidine hydrochloride (GdnHCl) passes through essential unfolding of the protein. This means that inactivated actin should be consider...

To improve our understanding of the contributions of different stabilizing interactions to protein stability, including that of residual structure in the unfolded state, the small sweet protein monellin has been studied in both its two variant forms, the naturally occurring double-chain variant (dcMN) and the artificially created single-chain variant (scMN). Equilibrium guanidine hydrochloride-...

When Goldblatt and coworkers (1) in 1934 first announced that a significant and sustained elevation in arterial blood pressure could be produced in dogs by constriction of the renal arteries they also reported a few experiments in which the level of the guanidine bases in the blood stream of these animals had been followed. Although their preliminary studies indicated that these substances were...

It is unknown why the potencies and efficacies of long-chained guanidine-type histamine H2-receptor (H2R) agonists are lower at the H2R of human neutrophils than at the H2R of the guinea pig atrium. To elucidate these differences, we analyzed fusion proteins of the human H2R (hH2R) and guinea pig H2R (gpH2R), respectively, and the short splice variant of Gsalpha (GsalphaS) expressed in Sf9 cell...