background and purpose: by using advanced detection/identification methods, the list of emerging uncommon opportunistic yeast infections is rapidly expanding worldwide. our aim in the present study was sequence-based species delineation of previously unidentified yeasts obtained from a clinically yeast collection. materials and methods: a total of twenty three out of the 855 (5.7%) yeast isolat...

During a previous longitudinal study, performed on four farrow-to-finish farms (A to D), samples were taken from twelve sows, their offspring, and the environment on various occasions over six months to study the MRSA presence. During the present study, a selection of the obtained MRSA isolates were typed by multiple-locus variable-number tandem-repeat analysis (MLVA), Pulsed Field Gel Electrop...

genes. The PCR-based replicon typing method showed that this blaNDM-1-positive plasmid belonged to the IncHI incompatibility group. Genetic structures surrounding the blaNDM-1 gene were investigated using a PCR mapping approach and sequencing. The blaNDM-1 gene was immediately bracketed by a truncated insertion sequence ISAba125 upstream and the bleomycin resistance gene bleMBL downstream. This...

Genetic Programming is increasing in popularity as the basis for a wide range of learning algorithms. However, the technique has to date only been successfully applied to modest tasks because of the performance overheads of evolving a large number of data structures, many of which do not correspond to a valid program. We address this problem directly and demonstrate how the evolutionary process...

abstract background and objective: a high level of homogeneity observed within all bacteria in the mycobacterium tuberculosis complex makes a property that seriously challenges traditional biochemical-based identification methods of these pathogens in the laboratory. the work presented here was conducted to characterize mycobacterium tuberculosis complex isolates in golestan, northern iran. mat...

Daqu is a spontaneous, solid-state cereal fermentation product used for saccharification and as starter culture Chinese Baijiu production. Bacillus Acinetobacter , two dominant microbial genera in Daqu, produce enzymes organic acids that influence the quality. However, there are no rapid analytical methods detecting . We designed primers specific to perform genetic comparisons using 16 S rRNA. ...

introduction: methicillin-resistant staphylococcus aureus (mrsa) is responsible for an increasing number of serious hospital- and community-acquired infections. usa300 is known to be the most common cause of community-acquired infections, but recently there have been some reports on hospital-acquired infections caused by this strain. methods: totally 171 isolates of s. aureus were collected fro...

Various genetic markers have been exploited for fingerprinting the Mycobacterium tuberculosis complex (MTBC) in molecular epidemiological studies, mainly through identifying restriction fragment length polymorphisms (RFLP). In large-scale studies, RFLP typing has practical processing and analysis limitations; therefore, attempts have been made to move towards PCR-based typing techniques. Spolig...