background: relapsing fever caused by borrelia persica , is an acute tick-borne disease which is transmitted by soft ticks of ornithodoros tholozani to human.     methods: value of pcr and xenodiagnosis for detection of b. persica in o. tholozani ticks was compared. sixty-four bor­relia -free ticks were fed on infected guinea pigs and used for the experiments. for xenodiagnosis, a group of 32 t...

Breast cancer is a complex genetic disease that has an average annual incidence of two million people and the second leading cause of death among women all over the world. Micro-RNAs are consistently reported to regulate gene expression in all cancers. The present study, the correlation between the expression of hsa-miR-490-5p and nuclear factor of activated T-cells 5 (NFAT5) in breast cancer w...

Introduction: Spirochetes of Borrelia can be visualized directly in infected ticks by dark-field microscopy. Inoculation of in phosphate buffered saline (PBS) suspension of ground Argasid soft ticks to susceptible animals or allowing the ticks to feed on the same species followed by microscopic examination of the animals’ blood have also been practiced. With the advent of molecular method...

in the present study, an attempt was made to develop galactosylated albumin nanoparticles of cimetidine for treatment of acetaminophen induced hepatotoxicity. by developing the galactosylated nanoparticulated delivery of cimetidine the required action of drug at the target site i.e at liver can be provided. the advantage of targeting helps to reduce the systemic side effects which may be occur ...

legionella pneumophila ( l. pneumophila ) is a gram-negative bacterium which causes ‎legionnaires’ disease as well pontiac fever. the legionella infections in patients suffering from ‎neutropenia- as a common complication of cancer chemotherapy- can distribute rapidly. we ‎aimed to detection of l. pneumophila in haematological malignancy suffering patients with ‎nuetropenic fever by targeting t...

Production of knockout mice using targeted embryonic stem cells (ESCs) is a powerful approach for investigating the function of specific genes in vivo. Although the protocol for gene targeting via homologous recombination (HR) in ESCs is already well established, the targeting efficiency varies at different target loci and is sometimes too low. It is known that knockdown of the Bloom syndrome g...

Enhancers are short regulatory regions (50-1500 bp) of DNA that control the tissue-specific activation of gene expression by long distance interaction with targeting gene regions. Recently, genome-wide identification of enhancers in diverse tissues and cell lines was achieved using high-throughput sequencing. Enhancers have been associated with malfunctions in cancer development resulting from ...

Ends-in and ends-out gene replacement approaches have been successfully used to disrupt Drosophila genes involved in a variety of biological processes. These methods combine double-strand breaks and homologous recombination to replace a targeted chromosome region with a designed DNA sequence. Unfortunately, these methods require large numbers of single animal crosses, making them both time cons...

Production of knockout mice using targeted embryonic stem cells (ESCs) is a powerful approach for investigating the function of specific genes in vivo. Although the protocol for gene targeting via homologous recombination (HR) in ESCs is already well established, the targeting efficiency varies at different target loci and is sometimes too low. It is known that knockdown of the Bloom syndrome g...