BACKGROUND Early detection of melanoma is of great importance to reduce mortality. Discovering new melanoma biomarkers would improve early detection and diagnosis. Here, we present a novel approach to detect volatile compounds from skin. METHODS AND FINDINGS We used Head Space Solid Phase Micro-Extraction (HS-SPME) and gas chromatography/mass spectrometry (GC/MS) to identify volatile signatur...

major salivary gland tumors are uncommon and the exact nature of these tumors is not obvious. this study was carried out to compare the histological results of intraoperative frozen sections against those of permanent reports for major salivary glands masses. one hundred thirty-nine patients with major salivary gland masses who were candidates for surgery underwent an intraoperative frozen sect...

The primary therapeutic goal for the oncology patient is survival. Recent advances in diagnoses and treatment of neoplasia have resulted in an ever-increasing number of patients being cured and resuming a normal life. However, recognized side effects of treatments used to eradicate malignancies are temporary or permanent sterility. The cryopreservation of ovarian tissue harvested before cancer ...

This study explores the hypothesis that combining the minimally invasive surgical techniques of cryosurgery and pulsed electric fields will eliminate some of the major disadvantages of these techniques while retaining their advantages. Cryosurgery, tissue ablation by freezing, is a well-established minimally invasive surgical technique. One disadvantage of cryosurgery concerns the mechanism of ...

One of the criteria of selection for skin sparing mastectomy (SSM) with nipple areola complex (NAC) preservation is to exclude the neoplastic involvement of subareolar tissue (NAC base) in order to minimize the possibility of local recurrence. The most common way to assess the possible neoplastic involvement is intraoperative frozen section of the NAC base tissue. Because of its limitations, pa...

The first demonstration of intracytoplasmic virus by negative staining was that of Horne and Nagington (9) who subjected poliovirus-infected cells to freezing and thawing and negatively stained a suspension of the fragments. Dales (5) examined the attachment of some viruses to the cell surface by the negative staining of whole mounts of cells subjected to osmotic shock. Whittaker and Horne (15)...

High-density SNP microarrays provide insight into the genomic events that occur in diseases like cancer through their capability to measure both LOH and genomic copy numbers. Where currently available methods are restricted to the use of fresh frozen tissue, we now describe the design and validation of copy number measurements using the Illumina BeadArray platform and the application of this te...

miRNAs are small molecules involved in gene regulation. Each tissue shows a characteristic miRNAs epression profile that could be altered during neoplastic transformation. Glioblastoma is the most aggressive brain tumour of the adult with a high rate of mortality. Recognizing a specific pattern of miRNAs for GBM could provide further boost for target therapy. The availability of fresh tissue fo...

Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry Imaging (MSI) is fast becoming an industry leading technique as a means of investigating the distributions of protein and peptide molecules directly from sections of tissue. Developing protocols for the analysis of FFPE tissue opens up numerous opportunities for novel biomarker discovery, due to the large number of tissue ban...