OBJECTIVES To demonstrate mucosal biofilms in human tissue by direct visualization of bacteria and glycocalyx using confocal laser scanning microscopy with double fluorescent staining on tonsils and to compare the findings with the results of scanning electron microscopy analysis. DESIGN Prospective study. SETTING Tertiary university-based referral center. PATIENTS Twenty-four tonsils wer...

A confocal laser microscope was used to analyze the localization pattern of dystrophin along the sarcolemma in guinea pig skeletal muscle fibers. Hind leg muscles of the normal animals were freshly dissected and frozen for cryostat sections, which were then stained with a monoclonal antidystrophin antibody. In confocal laser microscopy, immunofluorescence staining in relatively thick sections c...

The Materials Genome Initiative (MGI) is accelerating the pace of advanced materials development by integrating high-throughput experimentation, database construction, and intelligence computation. Live-cell imaging agents, such as fluorescent dyes, are exemplary candidates for MGI applications two reasons: i) they essential in visualizing cellular structures functional processes, ii) unclear r...

OBJECTIVES Ever since the discovery of Mycobacterium tuberculosis in 1882, many diagnostic methods have been developed. However "The gold standard" for the diagnosis of tuberculosis (TB) is still the demonstration of acid fast Bacilli (AFB) by microscopic examination of smear or bacteriological confirmation by culture method. MATERIALS AND METHODS In suspected 75 patients with active pulmonar...

The Ziehl-Neelsen stain is compared with three alternative methods of staining tubercle bacilli in paraffin sections: Fite's method (1938); a modification by Armstrong and Price (1947) of Fite's method; and a fluorescent method using the auramine-phenol stain. The fluorescent method was found to be the most effective of the four methods and its use is recommended as a routine tool in diagnostic...