نتایج جستجو برای: explant technique

تعداد نتایج: 614595  

St. John’s wort is a traditional medicinal plant that has been used from ancient time for the treatment of neurological and psychiatric diseases. A successful in vitro propagation system for the St. John’s wort was optimized. Different explant type (single node and  stem cutting with two nodes) were used in Murashige and Skoog (MS) medium in different concentrations (MS full, 3/4 MS and 2/3 MS)...

2009
Margaret Wan Nar Wong Wai Ting Lui Sai Chuen Fu Kwong Man Lee

BACKGROUND AND PURPOSE Previous studies on the culture of human tenocytes have shown that dexamethasone and triamcinolone reduce cell viability, suppress cell proliferation, and reduce collagen synthesis. However, such cell cultures lack the extracellular matrix and three-dimensional structure of normal tendons, which affects their response to stimuli. We established a human tendon explant cult...

Journal: :Journal of cell science 1994
E van der Zee V Everts K Hoeben W Beertsen

The effect of interleukin-1 alpha (IL-1 alpha) and murine epidermal growth factor (EGF) on incorporation of endogenously produced collagenase in the extracellular matrix of soft connective tissue was studied in an in vitro model system using periosteal explants obtained from rabbit calvariae. Immunohistochemical analysis indicated the highest level of collagenase in explants cultured for 72 hou...

Journal: :The Journal of Thoracic and Cardiovascular Surgery 2006

Journal: :European Journal of Vascular and Endovascular Surgery 2017

Journal: :Brazilian Journal of Development 2023

Use of light-emitting diodes (LEDs) in strawberry vitro multiplication The refinement plant tissue culture techniques is great importance for optimizing rates and improving the quality micropropagated changes, either or latest models light sources light..The objective this work was to evaluate effect type on Tudla cultivar. Strawberry sprouts were grown 30 days MS medium plus 100 mg L-1 myo-ino...

2017
Abhinav Reddy Kethiri Sayan Basu Sachin Shukla Virender Singh Sangwan Vivek Singh

PURPOSE Simple limbal epithelial transplantation (SLET) and cultivated limbal epithelial transplantation (CLET) are proven clinical techniques for treating limbal stem cell deficiency (LSCD). However, the ideal size and number of the limbal explants required for transplantation has not been clearly elucidated. This in vitro study aimed to determine the optimal limbal explant size required for c...

Journal: :Journal of engineering technology and applied sciences 2022

Tissue culture applications help mass production for many plant species, especially horticultural plants, ornamental medicinal and aromatic plants. Therefore, optimization of techniques is very important. In this study, the effects different Murashige Skoog (MS) salt levels (25%-200%) on in vitro Staurogyne repens (Nees) Kuntze were investigated. The shoot tip was used as explant. Different MS ...

Journal: :Gut 1999
Y Sirivatanauksorn V Sirivatanauksorn S Bhattacharya B R Davidson A P Dhillon A K Kakkar R C Williamson N R Lemoine

BACKGROUND Hepatocellular carcinoma (HCC) arising in cirrhosis is frequently multifocal. Whether HCC develops monoclonally or multiclonally is an unresolved question. Of the multiple tumour nodules present in many patients, it has not been established whether the smaller lesions represent intrahepatic metastases or de novo cancers. AIMS To assess the degree of genomic heterogeneity in synchro...

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