نتایج جستجو برای: explant technique
تعداد نتایج: 614595 فیلتر نتایج به سال:
St. John’s wort is a traditional medicinal plant that has been used from ancient time for the treatment of neurological and psychiatric diseases. A successful in vitro propagation system for the St. John’s wort was optimized. Different explant type (single node and stem cutting with two nodes) were used in Murashige and Skoog (MS) medium in different concentrations (MS full, 3/4 MS and 2/3 MS)...
BACKGROUND AND PURPOSE Previous studies on the culture of human tenocytes have shown that dexamethasone and triamcinolone reduce cell viability, suppress cell proliferation, and reduce collagen synthesis. However, such cell cultures lack the extracellular matrix and three-dimensional structure of normal tendons, which affects their response to stimuli. We established a human tendon explant cult...
The effect of interleukin-1 alpha (IL-1 alpha) and murine epidermal growth factor (EGF) on incorporation of endogenously produced collagenase in the extracellular matrix of soft connective tissue was studied in an in vitro model system using periosteal explants obtained from rabbit calvariae. Immunohistochemical analysis indicated the highest level of collagenase in explants cultured for 72 hou...
Use of light-emitting diodes (LEDs) in strawberry vitro multiplication The refinement plant tissue culture techniques is great importance for optimizing rates and improving the quality micropropagated changes, either or latest models light sources light..The objective this work was to evaluate effect type on Tudla cultivar. Strawberry sprouts were grown 30 days MS medium plus 100 mg L-1 myo-ino...
PURPOSE Simple limbal epithelial transplantation (SLET) and cultivated limbal epithelial transplantation (CLET) are proven clinical techniques for treating limbal stem cell deficiency (LSCD). However, the ideal size and number of the limbal explants required for transplantation has not been clearly elucidated. This in vitro study aimed to determine the optimal limbal explant size required for c...
Tissue culture applications help mass production for many plant species, especially horticultural plants, ornamental medicinal and aromatic plants. Therefore, optimization of techniques is very important. In this study, the effects different Murashige Skoog (MS) salt levels (25%-200%) on in vitro Staurogyne repens (Nees) Kuntze were investigated. The shoot tip was used as explant. Different MS ...
BACKGROUND Hepatocellular carcinoma (HCC) arising in cirrhosis is frequently multifocal. Whether HCC develops monoclonally or multiclonally is an unresolved question. Of the multiple tumour nodules present in many patients, it has not been established whether the smaller lesions represent intrahepatic metastases or de novo cancers. AIMS To assess the degree of genomic heterogeneity in synchro...
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