نتایج جستجو برای: denaturing

تعداد نتایج: 4945  

2009
V. Renouf P. Strehaiano A. Lonvaud - Funel

• These authors studied the microorganism population present at every step of the winemaking process in 3 estates located in 3 Bordeaux appellations: Libournais, Graves, and Medoc. For each estate, they started inventorying the microorganisms present at the vineyard level, continued with those present in the water resulting from cleaning the cellar equipment, and ended by analyzing the microorg...

Journal: :Journal of environmental sciences 2008
Yi Liu Feng Wang Siqing Xia Jianfu Zhao

In this study, the authors have investigated the effects of various factors on both aerobic and anaerobic degradation of 4-t-octylphenol (4-t-OP) in granular sludge. In comparison, the aerobic degradation rate was much higher than that of anaerobic degradation. The optimal pH values for 4-t-OP degradation in granular sludge were 9 and 7 under aerobic and anaerobic conditions, respectively. And ...

Journal: :Journal of microbiological methods 2004
Danilo Ercolini

Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting was recently introduced into food microbiology. This paper describes the technique and reports on the state-of-the-art application of this technique to food and food-related ecosystems. Applications of PCR-DGGE in several fields of food microbiology are reviewed: the identification of microorganisms isol...

2016
Md Rokunuzzaman Yumiko Ueda Li Chen Sota Tanaka Kouhei Ohnishi

Soil bacterial community structures in terraced rice fields and abandoned lands in a hilly and mountainous area were analyzed using 16S rRNA gene sequences. The DGGE band patterns of each soil were similar. Based on pyrosequencing data, the richness and diversity of bacterial species were slightly higher in paddy fields than in other soils. A beta-diversity analysis clearly indicated that the b...

Journal: :ACS applied polymer materials 2022

The development of environmentally friendly materials and processes is a major issue that concerns all industrial sectors, including microelectronics. aim this study to demonstrate the possibility using chitosan-based photoresists for microelectronic applications on silicon by 193 nm photolithography. photopatterning chitosan films demonstrated analyzed different spectroscopy microscopy techniq...

Journal: :IOP conference series 2023

Abstract The hydrogen content of the two sludge samples namely TDS and PLS were increased after heat shock pretreatment from 239.23 ± 10.23 mL/L to 663.87 15.99 at 80°C 173.61 17.21 496.74 30.21 60°C, respectively. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) indicated that Enterococcus Clostridium dominant bacteria in consortium. Two strains successfully isolate...

2012
Susan Bullman Daniel Corcoran James O’Leary Deirdre Byrne Brigid Lucey Roy D. Sleator Stephen L.W. On Angela J. Cornelius

Using PCR-denaturing gradient gel electrophoresis, we examined 49 fecal samples from healthy volunteers and 128 diarrhea specimens to assess the distribution of Epsilonproteobacteria that might be routinely overlooked. Our results suggest that certain taxa that are not routinely examined for could account for a proportion of diarrhea of previously unknown etiology.

Journal: :Mutation research 1999
A Patiño-García E Sotillo-Piñeiro C Modesto L Sierrasesúmaga

We have designed a new PCR-DGGE technique that enables detection of base changes in the TNF-alpha gene promoter. Screening of 130 samples from Spanish children has shown that this technique accurately detects the altered band patterns induced by the presence of the polymorphisms at positions -376, -308, -238 and -163 of the promoter sequence. Although further analysis are needed to fully charac...

2013
Yi Zhang Gaochan Wang Yupeng Wu Hui Zhao Yufeng Zhang Zhenjun Sun

In order to test if the intestinal bacteria play an important role in antibacterial ability of earthworm, we chose Escherichia coli O157:H7, an anthropozoonosis pathogen, as a biological stressor and studied the change of intestinal bacteria community of earthworm by PCR-DGGE analysis. Results showed that the pathogen merely existed 1 3 days, then almost disappeared after through the earthworm’...

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