An iron-fortified whey protein concentrate (Fe-WPC) was prepared by addition of ferric chloride to concentrated whey. A large part of the iron in the Fe-WPC existed as complexes with proteins such as β-lactoglobulin. The bioavailability of iron from FeWPC was evaluated using iron-deficient rats, in comparison with heme iron. Rats were separated into a control group and an irondeficiency group. ...

Weaning rats were divided into two groups, one group being fed a vitamin E-deficient diet, and the other an alpha-tocopherol-containing (50 mg/kg) control diet. Rats were killed at 1, 2, 3 and 4 months of feeding. The following results were obtained. 1. Both plasma and liver alpha-tocopherol levels decreased greatly by feeding vitamin E-deficient diet for 1 month, and thereafter decreased gradu...

The extension of lifespan due to reduced insulin-like growth factor 1 (IGF-I) signaling in mice has been proposed to be mediated through alterations in metabolism. Previously, we showed that mice homozygous for an insertion in the Igf1 allele have reduced levels of IGF-I, are smaller, and have an extension of maximum lifespan. Here, we tested whether this specific reduction of IGF-I alters gluc...

Effects of iron deficiency on the hepatocyte were studied quantitatively in the rat by combining ultrastructural and biochemical techniques. After 3-8 wk of an iron-deficient diet, the percentage of cytoplasm occupied by mitochondria increased progressively compared with complete diet values. The increment resulted primarily from an enlargement of individual mitochondria rather than from an inc...

The current trial was conducted to investigate effects of tryptophan (Trp) supplementation in crude protein (CP) deficient diets on performance, gut development and immune responses in broiler chickens. A total of 420 day-old broiler chicks (Ross 308) were assigned to one of 6 dietary treatments, ‎comprising 5 replicates in a completely randomized design including: 1) ‎control diet (CTL) (diet ...

Anemia can be induced by dietary iron deficiency, as well as by hemorrhagia. It may also be associated with changes in lipid metabolism. However, no global analysis detailing the consequences of iron deficiency in the liver has yet been conducted. Since the liver is a metabolically important organ and also a major iron-storing organ, we performed a comprehensive transcriptome analysis to determ...

Adipose tissue (AT) inflammation is a central mechanism for metabolic dysfunction in both diet-induced obesity and age-associated obesity. Studies in diet-induced obesity have characterized the role of Fetuin A (Fet A) in Free Fatty Acids (FFA)-mediated TLR4 activation and adipose tissue inflammation. However, the role of Fet A & TLR4 in aging-related adipose tissue inflammation is unknown. In ...

Rats were separated into four groups and four different liquid diets were given to each group. Group 1: thiamine-sufficient diet with no ethanol, group 2: thiamine-sufficient diet with ethanol, group 3: thiamine-deficient diet with no ethanol, group 4: thiamine-deficient diet with ethanol. After four weeks, all rats were fasted for 24 hr and then ethanol was given orally to every rat. After one...

Rats were raised after weaning on a vitamin D-deficient diet which used whole wheat and casein as the major protein source. For at least the first year of life, plasma calcium concentrations of these rats were the same as those of vitamin D-replete rats, and the rate of growth was normal for at least 6 months. The following evidence establishes the vitamin D deficiency of the rats (both male an...

OBJECTIVES Nonalcoholic steatohepatitis (NASH) is an advanced stage of non-alcoholic fatty liver disease (NAFLD) from steatosis. Methionine and choline are important amino acids play a key role in many cellular functions. Glycine is a non-essential amino acid having multiple roles in many reactions. This study aimed to investigate liver damage induced by feeding male albino rats either methioni...