A comparative study of in vitro seed germination of two endangered orchid species, viz. Cymbidium elegans Lindl. and Dendrobium densiflorum Lindl. ex Wall., was carried out on Murashige and Skoog’s (MS) medium, supplemented with different concentrations and combination of 6-benzylaminopurine (BAP) and á-Naphthalene acetic acid (NAA). The hormone-free MS medium and MS medium supplemented with va...

Shoot multiplication of Bupleurum distichophyllum was achieved from the nodal and shoot tip explants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum explant response was from axillary shoots and the highest number of shoots per explant was obtained on MS fortified with 1.0 mg/l BAP. The highest degree of axillary shoot proliferation was fou...

background and objectives: grape has high nutritional value and it is an economically important horticultural plant in the world. iran has seventh producer grape rank in the world. today tissue culture is one of technique which can be used in grape propagation and breeding. material and method: in order to optimization of callus induction and micropropagation of grape in vitro condition, this i...

The present study was planned to enable in vitro conservation of Vanda testacea, a highly medicinal orchid species through asymbiotic seed germination technique Mitra medium supplemented with cytokinins (Kn - 4.65 μM, BAP 4.44 μM), and auxin (NAA- 5.37 μM). frequency initiation higher NAA augmented seedlings developed 12.50 ± 0.50 weeks. Coconut water (20%) proved optimum for the multiplication...

The morphogenic potential and free-radical scavenging activity of the medicinal plant, Silybum marianum L. (milk thistle) were investigated. Direct root regeneration and indirect organogenesis and preliminary phytochemical, antibacterial studies of its callus. From leaf explants the highly significance result was found on the NAA at 2 mg/l and KIN at 0.2 mg/l for the direct root induction and i...

MS liquid medium supplemented with 3 mg/l NAA and 1 mg/l BAP with pH 5.7 was used for suspension cultures. Suspension cultures subjected to biotic elicitors Aspergillus niger, Fusarium oxysporium, Rhizopus oryzae and Penicillium notatum induced increase in forskolin content. Among all the elicitors tried Aspergillus niger proved to be best elicitor at 5% CF which was 6 times higher than the con...

In the present study, small segments of cotyledon, apical bud, hypocotyl, and root of 10-day-old seedlings were isolated and transferred to Murashige and Skoog (MS) base medium with different treatments of phytohormones. Two media were used for apical bud culture: MS with indole-3-acetic acid (IAA) and kinetin (Kin) (1 mg/l) and the other medium, MS with double vitamin of MS and 6-benzylaminopu...

An efficient procedure for shoot regeneration was obtained by endosperm culture in Actinidia deliciosa cv. Hayward. Mature endosperm cultured on MS medium supplemented with 2 mg/l 2,4-D and 5 mg/l kinetin developed callus with 80% efficiency. Callus was transferred on MS medium containing different plant growth regulators (2,4-D, TDZ, IAA, NAA, BAP, kinetin, 2iP) for regeneration. There were si...