This study, it was aimed to be enabling to use of fish scale as a bio-indicator for heavy metal pollution that is gradually increase in natural waters. For this purpose scaled carps, Cyprinus carpio (Linnaeus, 1758) were exposed to 7.5, 15, 30 and 60 μg L concentrations of trivalent chromium for 21 day and deformities appearing on the scales were examined under electron microscopy. It was found...

Cyprinid herpesvirus 3 (CyHV-3), also called koi herpesvirus (KHV), is the aetiological agent of a fatal disease in carp and koi (Cyprinus carpio L.), referred to as koi herpesvirus disease. The virus contains at least 40 structural proteins, of which few have been characterised with respect to their immunogenicity. Indirect immunofluorescence assays (IFAs) using two epitope-specific monoclonal...

Spring viraemia of carp (SVC) is a viral disease that mainly affects carp Cyprinus carpio and other cyprinid fish, causing severe economic losses. Rapid detection and identification of spring viraemia of carp virus (SVCV) is crucial for effective disease management. Recent advances in nanoscience are having a significant impact on many scientific fields, especially biodiagnostics, where a numbe...

Here we show the cloning and characterization of a novel homolog of prepro C-RFa cDNA from Cyprinus carpio. The deduced preprohormone precursor of 115 amino acids leads to a mature bioactive peptide of 20 amino acids with identical sequence to other teleost C-RFa. Modeling of the mature C-RFa peptide highlighted signifi cant similarity to homologous human PrRP20, specifi cally the conserved amp...

It has been hypothesized that, when engulfing food mixed with inorganic particles during benthic feeding, cyprinid fish use protrusions of tissue from the palatal organ to retain the food particles while the inorganic particles are expelled from the opercular slits. In crossflow filtration, the particle suspension is pumped parallel to the filter surface as filtrate exits through the filter por...

In vivo measurements of the cardiovascular responses of anoxia-tolerant teleosts to severe prolonged hypoxia are limited. Here, we report the first direct measurements of cardiac output (Q), heart rate (f(H)) and stroke volume during prolonged severe hypoxia (<0.3 mg O(2) l(-1)) in common carp (Cyprinus carpio L.) that had been acclimated to 6, 10 and 15 degrees C. While routine Q and f(H) valu...

IgM antibody diversity induced by viral infection in teleost fish sera remains largely unexplored despite several studies performed on their transcript counterparts in lymphoid organs. Here, IgM binding to microarrays containing ~20,000 human proteins was used to study sera from carp (Cyprinus carpio) populations having high titers of viral neutralization in vitro after surviving an experimenta...

There are a variety of approaches to identify groups of genes that change in expression in response to a particular stimulus or environment. We here describe the application of suppression subtractive hybridization (SSH) for isolation and identification genes in the brain of common carp (Cyprinus carpio) under cold temperatures. The materials were prepared through cooling the hybrid F2 of purse...

Research is ongoing to develop screening and testing programmes for endocrine disrupting effects of new chemicals and in the focus of this development are the fish test species common carp (Cyprinus carpio). In this study we have developed quantitative enzyme linked immunosorbent assays (ELISA) for VTS in common carp. The working range of the ELISA was 11.25 to 2000 ng mL(-1) (75-25% specific b...

Carp edema virus (CEV), the causative agent of 'koi sleepy disease' (KSD), appears to be spreading worldwide and to be responsible for losses in koi, ornamental varieties of the common carp Cyprinus carpio. Clinical signs of KSD include lethargic behaviour, swollen gills, sunken eyes and skin alterations and can easily be mistaken for other diseases, such as infection with cyprinid herpesvirus ...