The morphological analysis of the cytopathic effect on MDCK cell monolayers and hamster cornea and qualitative and quantitative analyses of conditioned medium and proteases were evaluated and compared between two strains of Acanthamoeba genotype T4. Further than highlighting the biological differences found between both strains, the most important observation in this study was the fact that pro...

Polyvinyl alcohol (PVA) is a biocompatible, transparent hydrogel with physical strength that makes it promising as a material for an artificial cornea. In our previous study, type I collagen was immobilized onto PVA (PVA-COL) as a possible artificial cornea scaffold that can sustain a functional corneal epithelium. The cellular adhesiveness of PVA in vitro was improved by collagen immobilizatio...

The morphologic appearance and time course of regenerating rabbit corneal epithelium was studied after 6.0 mm. corneal abrasions. The immediate response to injury was separation and thickening of basal and squamous epithelial cells at and near the wound margin. A variable number and distribution of polymorphonuclear leukocytes appeared on the basal lamina and regenerating epithelial edge at 3 h...

PURPOSE To describe the clinical and pathologic characteristics of a family with a congenital stromal dystrophy of the cornea and to identify the genetic basis for this disorder. METHODS All family members in three generations underwent ophthalmic examination. Stored corneal buttons were examined by transmission electron microscopy. Molecular genetic studies, including a genome-wide scan with...

Background/Objective: Hyperphosphatemia is the consequence of end stage renal failure. Inadequate control of serum phosphorus results in elevated Ca×P product with subsequent soft tissue deposition in the form of conjunctival and corneal calcification.  In this study, we evaluated the relationship of conjunctival and corneal calcification with secondary hyperparathyroidism in hemodialysis patie...

Background: Correlations between bone marrow, heart, kidney, liver, skin and lung transplant rejection or survival with human cytokine gene polymorphisms have been described. There are also reports about the role of cytokines and Tumor Necrosis Factors-Alpha (TNF-α) on corneal transplant in animal models. Further studies are needed to clarify the role of cytokines in corneal allograft rejection...

To fabricate donor corneal substitutes based on carbodiimide cross-linked porcine collagen, to study their in vitro and in vivo properties, and to elaborate new implantation techniques for the donor corneal collagen-based substitutes, this study had been performed. Bioengineered substitutes of corneal stroma (BSCS) were fabricated by cross-linking porcine type I collagen with 1-ethyl-3-(3-dimet...

The nature of the substrate that supports epithelial migration in vivo is of interest, particularly with respect to mechanisms of wound healing. Immunofluorescence and electron microscopy were used to search for common substrate components in prototype rabbit corneal wounds: epithelial scrape wounds, in which the corneal or conjunctival epithelium migrated over the denuded lamina densa of the c...

PURPOSE To describe the clinical outcome of amniotic membrane transplantation (AMT) for nontraumatic corneal perforations, descemetoceles, and deep ulcers. DESIGN Retrospective, noncomparative, interventional case series. PARTICIPANTS Thirty-four eyes of 33 consecutive patients operated on for nontraumatic corneal perforations or descemetoceles at four academic departments of ophthalmology....

PURPOSE This paper is mainly about biomechanical behavior of needle insertion into cornea, and proposes a failure criterion to simulate the insertion process which has attracted considerable attention due to its importance for the minimally invasive treatment. METHODS In the process of needle insertion into cornea, tiny and complex insertion force is generated due to contact between needle an...