The aim of this study was to investigate the use of photochimicals processes (photolysis, H2O2/UVLP and NaOCl/UVLP) to eliminate monochloramine compound using a Low Pressure Hg lamp as an irradiation source (254 nm). First, it was found that the direct photolysis treatment might be rate of depletion under UV dose at 8000 mj/m2). Besides, this degradation was greatly improved by H2O2/UVLP thank’...

The fungistatic and fungicidal activity of N-chlorotaurine (NCT), a long-lived oxidant produced by stimulated neutrophils, was investigated. Physiological concentrations (75-100 microM) of NCT showed clear fungicidal activity against a range of Aspergillus isolates. Moreover, killing by NCT was significantly increased in the presence of ammonium chloride, explained by the formation of monochlor...

Kinetics and mechanisms of As(III) oxidation by free available chlorine (FAC-the sum of HOCl and OCl-), ozone (O3), and monochloramine (NH2Cl) were investigated in buffered reagent solutions. Each reaction was found to be first order in oxidant and in As(III), with 1:1 stoichiometry. FAC-As(III) and O3-As(III) reactions were extremely fast, with pH-dependent, apparent second-order rate constant...

A radioimmunoassay procedure for determination of somatostatin immunoactivity in human plasma is described. Labelling of tyrosine-1-somatostatin is performed by chloramine T. Specific activity of the tracer is 1.34 mol iodine for 1 mol of somatostatin. The immunoreactivity is 85%. Antisera are produced in rabbits at a titre of 179 X 10(-12) mol somatostatin per ml antiserum. Human plasma is ext...

A rapid, simple, and sensitive 125I-postlabeling technique has been developed to allow detection of DNA-protein cross-links induced by environmental contaminants and carcinogens. This method is based on specific incorporation of 125I into tyrosine residues associated with DNA. Cultured Chinese hamster ovary cells were exposed to various crosslinking agents, e.g., UV light, K2CrO4, or NiCl2. DNA...

4-Deuterioglutamic acid was prepared by incubation in DzO of ammonium mesaconate with an extract of Clostri&urn tetanomorphum. In these extracts, methylaspartase catalyzed the formation of threo-3-methyl-L-aspartate-3-D, which was then rearranged to glutamate by the cobamide coenzyme-dependent glutamate mutase. The monodeuteriosuccinate obtained by chloramine-T oxidation of the glutamate showed...

The kinetics of oxidation cyclopentanol, cyclohexanol and cyclooctanol using Chloramine T in NaOH has been studied in the micellar phase using sodium dodecyl sulphate in the temperature range 30°C to 50°C to correlate the oxidation rate with the ring size of the cyclic alcohols. The effect of alcohol concentration, concentration of oxidising agent, ionic strength and temperature on the oxidatio...

We have used two methods for the preparation of a highly homogeneous insulin with high specific activity. After iodination with chloramine T, the labeled peptides were retained on a disposable Sep Pak cartridge and subsequently eluted. The eluted labeled insulins were further purified by either DEAE cellulose or high performance liquid chromatography (HPLC) to separate A14-125I- from A19-125I-i...

The disinfection by-product (DBP) formation was affected by the dissolved organic matter (DOM). Therefore, DOM properties and DBP potential of two most widely used source waters: groundwater (GW) surface water (SW), were comparatively studied in this work. results suggested that GW mainly consisted protein-like organics with smaller molecular weight (Mw) less than 3000 Da, while SW contained hu...