The nucleocapsid (N) gene of infectious bronchitis virus (IBV) strain X isolated in China was expressed in E. coli and was purified as a recombinant protein. An indirect ELISA assay (N-ELISA) for antibody detection was established using the purified recombinant nucleocapsid protein. Antigen coating conditions and serum dilution for the N-ELISA were optimized. The S/P ratio of the absorbency val...

Methods The study group included 106 HIV positive patients with history of heterosexual contact and the control group included 30 asymptomatic healthy individuals. The sera of patients were tested for HBsAg and antiHCV using third generation ELISA kits. The samples positive for first kit were retested for confirmation of results. The sera of control group were simultaneously screened for HIV, H...

Background: Blood transfusion improves health and saves lives. Safe blood must be ensured for our populations. Quality assurance is a process that includes set of coordinated activities in order to achieve the quality objective. Compliance with management rules medical biology laboratories requires verification methods prior their use. This study aimed verify on-site performance Enzyme Linked I...

Introduction: Leptospirosis is a zoonotic infection affecting humans. The main sources of are animal reservoir hosts and man the accidental host in disease transmission process. diagnosis usually made by microscopy, culture, molecular techniques, serological tests like ELISA, MAT (Microscopic Agglutination Test) MSAT (Macroscopic Slide Test). ELISA method to detect IgM antibodies used as good c...