A Phase II trial using interleukin 1alpha (IL-1alpha) and etoposide for patients with relapsed osteosarcoma (OS) was undertaken to assess the feasibility and tolerability of combination therapy with biotherapy and chemotherapy. Nine patients with histologically proven relapsed OS were treated with IL-1alpha immediately followed by etoposide daily for 5 days every 3 weeks. Surgical resection of ...

OBJECTIVE Operations coupled with cardiopulmonary bypass may provoke a systemic inflammatory response, and it has been suggested that this responses causes capillary leakage of proteins, edema formation, and even organ failure. However, capillary leak syndrome is mainly a clinical diagnosis and has not been verified as yet by actual demonstration of protein leakage from the circulation. We have...

Detection and quantitation of nucleic acids have gained much importance in the last couple of decades, especially in the post-human genome project era. Such processes are tedious, time consuming and require expensive reagents and equipment. Therefore, in the present study, we demonstrated a simple process for the separation and analysis of small DNA fragments using capillary electrophoretic amp...

We introduce sweeping capillary electrophoresis (SweepCE), a non-stopped-flow method for directly measuring the bimolecular rate constant of complex formation, and demonstrate its use for studying protein-DNA interaction. The capillary is prefilled with a solution of DNA, and electrophoresis is then carried out from a solution of the protein in a continuous mode. Because the electrophoretic mob...

Neuraminidase inhibitor assay requiring complicated step-by-step operation was successfully integrated into ”Single-Step” by using CPC sensor. We developed a combinable PDMS capillary (CPC) sensor for enzyme inhibition assay using the concave and convex type PDMS substrates. In the conventional enzyme inhibitor assay, complicated analytical procedures, such as mixing and dissolution, are requir...

This paper describes a procedure, based on competitive binding, for identifying tight-binding ligand(l)Ig a receptor in mixtures of equimolar ligand candidates using affinity capillary electrophoresis (ACE). Vancomycin and a small library of 32 peptides were used as a model syatem to illushate the procedureThis procedure should be applicable to peptide mixtures of greater complerity than the on...

The functional competence of each organ depends markedly on characteristics of its capillary bed, i.e. volume, structure of the capillary wall, rate of the blood flow, spatial arrangement and relationship to the neighboring structural components of the organ (e.g. epithelium, muscle tissue). The knowledge of the structure, quantity, arrangement and features of microvascular bed of the organ is ...

A method was validated for the determination of the 2 main components of bee venom, phospholipase A2 and melittin, by capillary electrophesis (CE). Optimum resolution and selectivity were attained with a running electrolyte of 150 mM phosphoric acid, pH 1.8. The repeatability and day-to-day reproducibility of the migration times were better than 0.36 and 2.8%, respectively. The repeatability an...

For many researchers, examining difficult DNA template sequencing is an essential part of evaluating a capillary electrophoresis system. Template regions that present increased average sequencing complexity are not uncommon. Most difficult templates contain a feature such as a GC-rich region or a hairpin loop that may inhibit the activity of DNA polymerase. Long homopolymer strings can also red...

Capillary electrophoresis (CE) coupled to electrospray mass spectrometry via sheathless interfacing has been applied to the analysis of mammalian metallothionein (MT) extracts. In a rabbit-liver extract, four (MT-2C, MT-2A, MT-2D and MT-2E) out of six known MT sub-isoforms were unambiguously identified under three CE-resolved peaks. A fourth peak was found to contain MT-1A and/or MT-2B, whose m...