Plants can regenerate from a variety of tissues on culturing in appropriate media. However, the metabolic shifts involved in callus formation and shoot regeneration are largely unknown. The metabolic profiles of callus generated from tomato (Solanum lycopersicum) cotyledons and that of shoot regenerated from callus were compared with the pct1-2 mutant that exhibits enhanced polar auxin transpor...

Cactus Mammillaria gracillis Pfeiff. (Cactaceae), cultivated in vitro, spontaneously switches from an organised to unorganised way of growth, producing a habituated organogenic callus which regenerates normal and hyperhydric shoots without the addition of any growth regulators. Tumour tissues, induced by A. tumefaciens wild strain B6S3 (tumour TW) and rooty mutant GV3101 (tumour TR), do not exp...

The present study was conducted to investigate the effects of different concentrations and combinations of plant growth regulators (PGRs) on callus induction of Tecomella undulata (Sm) Seem. The leaf segments were used as explants and cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of auxins such as NAA, 2,4-D, IAA and cytokikinins like BAP and KIN alone a...

this study was conducted to investigate the effects of in vitro and regeneration of lily (lilium longiflorum) bulb scale by different plant growth regulators in b5 medium in a completely randomized design with factorial arrangement and 4 replications. treatments consisted of indole-3-acetic acid (iaa) and 2,4-d in b5 medium in four concentrations (0, 0.5, 1, 2 mg/lit) and these were combined to...

Highly efficient in vitro regeneration system has been developed for Swertia lawii Burkill, an important herb used as substitute for Swertia chirayita. Shoot tips explants were cultured on MS medium with various phytohormones for multiple shoot production. The best shoot production frequency (100%) and maximum shoots (10.4 ± 0.8) were obtained on MS media containing TDZ (3.0 mg l-1) in combinat...

A mutagenesis programme was carried out using physical mutagens (gamma rays) on Cucumis melo cv. Bathasa. In irradiated seeds the number of shoots formed in the lower doses was significantly higher than the controls. Decrease in the number of shoots and shoot bud formation was observed with higher doses in all the explants studies. There was complete lethality in the 10, 15 and 20 kR doses. In ...

In this review, we report on the different plant growth regulators (PGRs) affecting callus induction, callus-mediated regeneration, organogenesis, somatic embryogenesis from explants and micropropagation through shoot tip nodal cultures in Turkey Berry (Solanum torvumSW). The fruits of guard against liver kidney damage, stop certain cancers, regulate blood sugar levels. They benefit digestion, ...

Abstract Rhizogenic, callus, and suspension cultures in vitro were obtained for Digitalis lanata their growth, cytophysiological biochemical characteristics investigated. The characterized by good growth (growth indexes I the range of 5–13). Suspension cell culture had a specific rate μ within 0.2–0.3 days –1 it was two-phase curve retardation during exponential phase). In cultures, study quali...