Six bacteriocinlike peptides (plantaricin A [PlnA], PlnE, PlnF, PlnJ, PlnK, and PlnN) produced by Lactobacillus plantarum C11 were detected by amino acid sequencing and mass spectrometry. Since purification to homogeneity was problematic, all six peptides were obtained by solid-phase peptide synthesis and were tested for bacteriocin activity. It was found that L. plantarum C11 produces two two-...

X-ray repair cross-complementing protein-1 (XRCC1)-de®cient cells are sensitive to DNA damaging agents and have delayed processing of DNA base lesions. In support of its role in base excision repair, it was found that XRCC1 forms a tight complex with DNA ligase IIIa and also interacts with DNA polymerase b (Pol b) and other base excision repair (BER) proteins. We have isolated wild-type XRCC1±D...

Hollow fiber liquid phase micro-extraction (LPME) of linear alkylbenzene sulfonates (LAS) from aqueous samples was studied. Ion pair extraction of C10, C11, C12 and C13 homologues was facilitated with trihexylamine as ion-pairing agent, using di-n-hexylether as solvent for the supported liquid membrane (SLM). Effects of extraction time, acceptor buffer concentration, stirring speed, sample volu...

The role of cytotoxic cells in in vivo immune functions such as allograft rejection is unknown. To begin to assess the function of cytolytic cells in vivo we have begun with cytolytic cell-specific functional molecules: we have isolated and characterized cytolytic cell-specific cDNA clones from cytolytic T cell clones, both encoding distinct serine esterases. The HF gene encodes a trypsin-like ...

Fifty-three algal cultures were isolated from freshwater lakes in Hainan, China. Four microalgal isolates were selected because they could be successfully cultivated at high density and demostrated a strong fluorescence after being stained with nile red. These cultures were identified as strains of Chlorella sp. C11, Chlamydomonas reinhardtii C22, Monoraphidium dybowskii C29, and Chlorella sp. ...

Predicted promoter regions of Milk vetch dwarf virus (MDV) components (C1-C11) were isolated and fused with a beta-glucuronidase (GUS) reporter gene and the characteristics of the promoters were examined. In transgenic tobacco calli, promoters of MDV C4 (encoding a cell-cycle link protein), C5 and C7 (both encoding unknown proteins), C6 (encoding a nuclear-shuttle protein) and C8 (encoding a mo...

Saturated (C2-C11) and unsaturated (C4-C5, C8) dicarboxylic acids were measured in arctic aerosol and surface snowpack samples collected during dark winter (February) and light spring (April-May) using a gas chromatography and gas chromatography/mass spectrometry. Their molecular distributions were characterized by a predominance of oxalic acid (C2), except for few spring snowpack samples that ...

The chemical and nutritional composition of the Longissimus thoracis muscle from 58 'Rubia Gallega' breed animals was measured to differentiate veal of weaned from unweaned calves. Fat, protein, ash, and moisture contents of muscle were determined by near infrared reflectance spectroscopy (NIRS) and the fatty acid (FA) profiles were determined by gas chromatography. Calves suckled their mothers...

The fungal iterative nonreducing polyketide synthases (NRPKSs) synthesize aromatic polyketides, many of which have important biological activities. The product template domains (PT) embedded in the multidomain NRPKSs mediate the regioselective cyclization of the highly reactive polyketide backbones and dictate the final structures of the products. Understanding the sequence-activity relationshi...