نتایج جستجو برای: b5 medium
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Citronella essential oil (CEO) has been reported as an excellent mosquito repellent; however, mild irritancy and rapid volatility limit its topical application. It was aimed to develop a nonirritant, stable, and consistent cream of CEO with improved residence time on skin using an industrial approach. Phase inversion temperature technique was employed to prepare the cream. It was optimized and ...
زمینه: سیتوکروم 1e4502p(1e2cyp) آنزیمی اصلی است که نقش عمده ای در فعال سازی و سمیت زدایی بسیاری از ژنوبیوتیک ها، سرطان زاها (کارسینوژن ها) و داروها ایفا می کند. بررسی انواع پلی مورفیسم در 1e2cyp به عنوان یک ضرورت اولیه خطر ابتلا به انواع سرطان در صورت تماس با ترکیبات کارسینوژن، یافته های متفاوتی داشته است. هدف از این بررسی تعیین فراوانی آللی و ژنوتیپی پلی مورفیسم b5*1e2cyp در جمعیت ایرانی و مقا...
Lycopene is present in a range of fresh fruits and vegetables, especially in the leaves of Barringtonia racemosa. The traditional lycopene extraction from the plant is being employed instead of an easy propagation technique like cell culture process from the leaf explants. We intend to assess how lycopene could be extracted via tissue culture under light (illuminance: 8,200 lux under white fluo...
The dehydrogenation reaction of cholest-7-en-3beta-ol (I) to cholesta-5,7-dien-3beta-ol (II) in the presence of NADH was studied in rat liver microsomes and in microsomal acetone powder preparations, using [3alpha-3H]cholest-7-en-3beta-ol. It was found that the reaction was inhibited by menadione, adenosine diphosphate, potassium ferricyanide, and cytochrome c while p-cresol had no effect. Thes...
This symposium summary, sponsored by the ASPET, was held at Experimental Biology 2015 on March 29, 2015, in Boston, Massachusetts. The symposium focused on: 1) the interactions of cytochrome P450s (P450s) with their redox partners; and 2) the role of the lipid membrane in their orientation and stabilization. Two presentations discussed the interactions of P450s with NADPH-P450 reductase (CPR) a...
Benzo[a]pyrene (BaP) is a human carcinogen that covalently binds to DNA after activation by cytochrome P450 (P450). Here, we investigated whether NADH:cytochrome b5 reductase (CBR) in the presence of cytochrome b5 can act as sole electron donor to human P450 1A1 during BaP oxidation and replace the canonical NADPH:cytochrome P450 reductase (POR) system. We also studied the efficiencies of the c...
The mode of action of triclopyr (3,5,6-trichloro-2-pyridinyloxyacetic acid), a broadleaf herbicide, is unknown. No data concerning the fate of triclopyr in plants have yet been published. This communication summarizes a comparison of the uptake and metabolism of triclopyr and 2,4-D (2,4dichlorophenoxyacetic acid) using cell suspension cultures of soybean, Glycine max, var. Harcor. Soybean cells...
Optimization of regeneration methods in cotton is necessary to improve and Successful production of transgenic cotton cultivars by tissue culture technique. This study was conducted to determine the genotypic effect on callus induction in cotton using 3 genotypes (Coker349, 4-S-4 and C1211) and Coker 312 selected and a study was carried out in Cotton Research Institute of Gorgan using a factori...
A sensitive, precise enzymic/spectrophotometric method for determining cytochrome b5 in small amounts of blood is described. Mean values for healthy individuals, ages 20 to 70 years, were 0.26 (SD 0.03) mumol per liter of erythrocytes or 0.87 (SD 0.14) nmol per gram of hemoglobin. We believe the assay is preferable to methods described hitherto, primarily because of its high sensitivity.
As a first-year medical student, you could count on the simplicity and elegance of the thyroid axis, because all its details had apparently been elucidated. Hypothalamic thyrotropin-releasing hormone (TRH) promoted the synthesis, release, and glycosylation of pituitary thyrotropin, thyroid-stimulating hormone (TSH), which in turn acted on the thyroid follicle, leading to the synthesis and relea...
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