Traumatic brain injury is considered as one of the main causes of morbidity and mortality worldwide. Apart from primary mechanical injury, Secondary injuries due to inflammation and apoptosis result in great neuronal damage. Current treatments are not able to regenerate the damaged part and prevent future sequels. Using human stem cells with self-assembling scaffolds may be promising in treatme...

To investigate the effect and molecular mechanism of vitexicarpin on biological behavior colorectal cancer cells is objective study. The human epithelial cell line Caco-2 were divided into control group, low, middle high dose groups vitexicarpin, plasmid cloning DNA-circ_0000419 DNA anti-microRNA-224 anti-microRNA-224-negative vitexicarpin+small interfering-circ_0000419 group vitexicarpin+micro...

AIM: To evaluate the effects of LIN28A (human) on high glucose-induced retinal pigmented epithelium (RPE) cell injury and its possible mechanism. METHODS: Diabetic retinopathy model was generated following 48h exposure to 30 mmol/L glucose (HG) in ARPE-19 cells. Quantitative real-time polymerase chain reaction (qRT-PCR) Western blot tested expression corresponding genes proteins. Cell viability...

Inappropriate DNA methylation of tumour suppressor genes can affect their expression and function, as such, has been a promising target for anti-cancer therapy. In gynaecological malignancy, ovarian cancer the highest associated rate mortality. This study investigated in vitro cytotoxicity four sesquiterpene lactones (SLs), including dehydroleucodine (Deh), alantolactone (Ala), costunolide (Cos...

The aim of this study was to investigate the activity apoptosis and autophagy in animals (cows, horses, donkeys, dogs cats) naturally infected with rabies by using immunohistochemistry, immunofluorescence, qPCR. mRNA transcript levels caspase-3, Bax, Bcl2 LC3B were determined Caspase-3 AIF immunopositivity not observed immunohistochemical immunofluorescence staining, whereas intensively compare...

Background: Spironolactone is a conventional drug widely in use for the treatment of heart failure and hypertension patients. On other side recent studies have reported spironolactone can prevent growth resistance cancer stem cells (CSCs), by inhibiting DNA double-strand break (DSB) repair; suggesting its potential application therapy. Objectives: Our study aimed at assessing cytotoxicity human...

Neutrophils are the primary inflammatory cell in smokers' lungs, but little is known about the ability of cigarette smoke to modulate neutrophil function. Neutrophils undergo caspase-3-dependent spontaneous, as well as phagocytosis-induced, apoptosis. This study investigated the ability of cigarette smoke extract (CSE) to alter neutrophil caspase-3 activity, apoptosis, and phagocytosis. CSE tre...

The AP-1 transcription factor c-Jun has been shown to be essential for stress-induced apoptosis in several models. However, the molecular mechanisms underlying the proapoptotic activity of c-Jun are poorly understood. We identify the apoptosis-antagonizing transcription factor (AATF) as a novel nucleolar stress sensor, which is required as a cofactor for c-Jun-mediated apoptosis. Overexpression...

To evaluate the role of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway in breast cancer cell survival and therapeutic resistance, we analyzed a panel of six breast cancer cell lines that varied in erbB2 and estrogen receptor status. Akt activity was constitutive in four cell lines and was associated with either PTEN mutations or erbB2 overexpression. Akt promoted breast cancer cell surviv...