The glycine transporter subtype 1 (GlyT1) is widely expressed in astroglial cells throughout the mammalian central nervous system and has been implicated in the regulation of N-methyl-D-aspartate (NMDA) receptor activity. Newborn mice deficient in GlyT1 are anatomically normal but show severe motor and respiratory deficits and die during the first postnatal day. In brainstem slices from GlyT1-d...

Purine biosynthesis by the 'de novo' pathway was demonstrated in isolated rat extensor digitorum longus muscle with [1-14C]glycine, [3-14C]serine and sodium [14C]formate as nucleotide precursors. Evidence is presented which suggests that the source of glycine and serine for purine biosynthesis is extracellular rather than intracellular. The relative incorporation rates of the three precursors w...

1. Extracts prepared from a variety of higher-plant tissues by ammonium sulphate fractionation were shown to catalyse the interconversion of glycine and serine. This interconversion had an absolute requirement for tetrahydrofolate and appeared to favour serine formation. 2. The biosynthesis of serine from glycine was studied in more detail with protein fractionated from 15-day-old wheat leaves....

The interactions between the glycine and glutamate binding sites of the NMDA receptor have been studied in outside-out patches and synapses from hippocampal neurons in culture using rapid drug application techniques. Desensitization of NMDA receptor-mediated currents elicited by glutamate in newly excised outside-out patches was reduced in the presence of saturating concentrations of glycine. T...

OBJECTIVE Accidental or intentional cyanide ingestion is an-ever present danger. Rapidly acting, safe, inexpensive oral cyanide antidotes are needed that can neutralize large gastrointestinal cyanide reservoirs. Since humans cannot be exposed to cyanide experimentally, we studied oral cyanide poisoning in rabbits, testing oral sodium thiosulfate with and without gastric alkalization. SETTING ...

UNLABELLED Previous findings from our group indicate that accumbal glycine receptors (GlyRs) are involved in mediating the dopamine (DA) activating effects of ethanol (EtOH), and that administration of glycine locally into the nucleus accumbens (nAc) reduces EtOH consumption in EtOH high-preferring rats. AIMS The present study examines the influence of a systemically administered glycine reup...

3-Amino-1-hydroxypyrrolid-2-one (HA-966) has been known for several years as an excitatory amino acid antagonist, acting principally at the N-methyl-D-aspartate (NMDA) receptor subtype. We report here that HA-966 blocks NMDA responses through a selective interaction with the glycine modulatory site present within the receptor complex. In radioligand binding experiments, HA-966 inhibited strychn...

abstract soybean (glycine max) is an important crop plant which contains a high amount of oil and protein. to evaluate the genetic diversity of 45 soybean genotypes growing in iran, two types of molecular markers (rapd and isj) were used. two sets of different primers were used to compare potential differentiation power of it (intron targeting) and et (exon targeting) primers. data obtained fro...

In this work, the different fuels (citric acid, glycine and urea) were used for solution combustion synthesis of CoFe2O4 powders. X-ray diffraction, Raman spectroscopy, electron microscopy and vibrating sample magnetometry techniques were employed for characterization of phase evolution, cation distribution, microstructure and magnetic properties of the as-combusted CoFe2O4 powders. Single phas...

The two membrane-associated protein components of the clostridial glycine reductase system were treated with detergents and separated by chromatography on DEAE-cellulose. Each was then further purified by chromatographic procedures. Protein B, obtained in highly purified form after loo-fold enrichment, contains a carbonyl group cofactor essential for its catalytic activity. The ability of this ...