نتایج جستجو برای: western bloting

تعداد نتایج: 183853  

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه پیام نور - دانشگاه پیام نور استان تهران - دانشکده علوم پایه 1389

جداسازی آماستیگوتها از زخم موشهای balb/c .کشت و تکثیر آماستیگوتها به طور in vitro.جداسازی غشاء آماستیگوتها و تجزیه آن به فراکسیونهای مختلف .انجامfusion و تولید آنتی بادی منوکلونال بر علیه آنتی ژن p4 غشایی آماستیگوت.جداسازی پروتئین p4 از غشاء آماستیگوت با sds-page و روش negative staining .خالص سازی پروتئین بدست آمده با elution . تشخیص پروتئین استخراجی به وسیله western blotting -ارزیابی پروتئین ب...

Journal: :iranian journal of virology 0
sf shams mashhad university of medical sciences (mums), mashhad, iran z arianpour blood transfusion organization research center, mashhad, iran f molahosseini-foomani blood transfusion organization research center, mashhad, iran hgh noorin blood transfusion organization research center, mashhad, iran

background and aims: hiv is spreading rapidly among people word wide. infection with this virus leads to immune suppression and finally acquired immune deficiency syndrome (aids).early hiv detection is depended on antibody screening against virus by enzyme linked immunosorbent assay (elisa).some confirmatory tests such as western blot and recombinant immunobloting assay (riba), are used to veri...

Journal: :journal of crop protection 2015
arezu lotfi masoud shams-bakhsh bagher yakhchali

serological methods are commonly used methods for detection of viruses. preparation of pure viral antigens is a crucial step in production of antibodies required for serological studies. in this research the gene encoding coat protein of a beet western yellows virus (bwyv) isolate from iran was amplified by pcr and was ligated into a bacterial expression vector (pet26b) to obtain pet-bwyv-cp cl...

Journal: :iranian journal of allergy, asthma and immunology 0
sedighe ebrahimpoor immunology department, pasteur institute of iran, tehran, ir-iran saeed-reza pakzad food and drugs control laboratory and research centre, ministry of health and medical education, tehran, ir-iran soheila ajdary immunology department, pasteur institute of iran, tehran, ir-iran

it is well accepted that in experimental model of leishmania major infection, balb/c mice mount a th2 response and produce igg1 predominantly whereas c57bl/6mice enhance th1 response with the biased production of igg2a antibodies. therefore, screening for parasite antigens on the basis of reactivity with sera from infected susceptible or resistant mice might be used for the identification of th...

Journal: :دراسات الادب المعاصر 0
سید أمیر محمود أنوار غلامرشا گلچین راد

it is obvious that literature means prose and poetry and makes no difference either by arabs or non – arabs. but symbolism in the arabic concept has emerged since the emergence of the dark era’s literature and is loaned from the specific characteristics of dark era’s lives and is based on two elements: brevity and indirect speech. since the symbolism has emerged like a literary school generally...

Journal: :iranian journal of veterinary research 2010
a. nematollahi r. jafari jozani

the intracellular parasite neospora caninumis prevalent in several countries and is increasingly recognized as an important cause of abortion and stillbirth in cattle. for characterizeing the tachyzoite antigens of neospora caninumin aborted cows, sera were obtained from 116 cows which were aborted in the third semester of the pregnancy period and had antibodies to neospora caninumin elisa. to ...

Journal: :archives of razi institute 2016
m. abdigoudarzi a. dadmehr f. golchinfar r. madani

partially fed (4 -5 days) h .a. anatolicum (iranian isolate) female adult ticks were used for preparation of antigens of midgut which are, the gut supernate antigen (gs), gut membrane antigen (gm), triton extracted gut membrane antigen ( tx-gm ) and sodium dodecyl sulphate treated triton extracted gut membrane antigen (sds-tx-gm). the new zealand white rabbits were immunized with gs, gm, tx- gm...

Journal: :journal of medical microbiology and infectious diseases 0
sedighe ebrahimpoor immunology department, pasteur institute of iran, pasteur ave., tehran, iran saeed reza pakzad vaccine potency and standardization section, food and drugs control laboratory research center, ministry of health and medical education, tehran, iran farideh mohasel hamedani immunology department, pasteur institute of iran, pasteur ave., tehran, iran soheila ajdary immunology department, pasteur institute of iran, pasteur ave., tehran, iran

in leishmania (l.) major-infected balb/c mice th2-type cells results in disease progression, whereas c57bl/6-infected mice mount a th1-type response, which leads to control of the infection. th2 response correlates with igg1 whereas, th1 response supports switching to igg2a subclasses. since igg isotype-dominated response depends on different cd4 + t cell subsets, we studied the antigenic profi...

Journal: :iranian journal of pharmaceutical research 0
omid gholami pharmacology department, faculty of medicine, shahid beheshti university of medical sciences, tehran, ir iran. mahmood jeddi-tehrani monoclonal antibody research center, avicenna research institute, acecr, tehran, ir iran. mehrdad iranshahi biotechnology research center and school of pharmacy, mashhad university of medical sciences, mashhad, ir iran. amir hassan zarnani nanobiotechnology research center, avicenna research institute, acecr, tehran, ir iran. seyed ali ziai pharmacology department, faculty of medicine, shahid beheshti university of medical sciences, tehran, ir iran.

umbelliprenin is a prenylated compound, which belongs to the class of sesquiterpene coumarins. it is extracted from dried roots of ferula szwitsiana collected from the mountains of golestan forest (golestan province, north of iran). induction of apoptosis in jurkat t-cll cells has been previously shown. in this study, effect of umbelliprenin on proapoptotic caspases (caspase-8 and -9) and antia...

The foundation of most intellectual discourse is Western-centric to the exclusion of other views. This includes ideal patterns of social interaction, preference for aesthetic, accepted norms and values, and the concepts of dignity, respectability, morality and ethics. Such a position is culturally myopic. Western cultural influence colors all of them including moral precepts. The assumption is ...

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