Reproducibility of reading "N-Multistix" dipsticks by a semi-automated urinalysis instrument (Ames' "Clini-Tek") has been described for artifically prepared samples. Glucose, ketone, urobilinogen, and nitrite showed high reproducibility (greater than 90%) for reading multiple samples at predetermined analyte concentrations. Determination of proteinuria showed the lowest proportion of false posi...

Gunn rats lack bilirubin UDP-glycosyltransferases, but diazo-negative derivatives of bilirubin have been described in their bile. In order to investigate this alternative disposal of bilirubin, crude bile samples from Gunn and Wistar rats were directly analysed by h.p.l.c. Besides bilirubin (in Gunn rats) or its glycosides (in Wistar rats), two major compounds were detected. A yellow one corres...

About 80 to 85 per cent of the fecal bile pigmients are believed to be derived from the hemoglobin of senescent erythrocytes that are destroyed in the reticuloendothelial system (1). It is not known, however, whether the hemoglobin of sequestered red cells is converted quantitatively to bilirubin and eventually to urobilinogen, or is in part metabolized to other products (2, 3). Earlier studies...

No urobilinogens are present in the feces or urine of germfree rats. After contamination of germfree animals with feces from conventional animals the exgermfree rats produced urobilins to the same extent as conventional animals on the same diet. The negative urobilin test turned positive in germfree animals infected with a single Clostridium-like microorganism isolated from the intestinal conte...

I. y EUGENE KAPLAN,* M.D. , AND S. ROBERT LEWIS, M.D. I N 1938, Josephsla.b reported a phenomenon occurring in children with hemolytic anemia: fecal urobilinogen excretion was observed to decrease following either blood transfusions, plasma injections or injections of concentrates of human or pig plasma. These decreases were of variable degree, occurred within a week of treatment, and lasted up...