The changes in urinary crystal properties in patients with calcium oxalate (CaOx) calculi after oral administration of potassium citrate (K3cit) were investigated via atomic force microscopy (AFM), scanning electron microscopy (SEM), X-ray powder diffractometry (XRD), and zeta potential analyzer. The AFM and SEM results showed that the surface of urinary crystals became concave, the edges and c...

DICARBOXYLATES ARE THE SUBSTRATES for oxidative phosphorylation, aka the Krebs cycle or citric acid cycle. Some of these substrates are synthesized within cells. However, epithelia with high metabolic needs (e.g., the small intestine and kidney) bring these substrates directly into cells. The plasma membrane of cells separates the outside world from the inside due to the hydrophobic core of thi...

The total and ultrafiltrable citrate of serum were measured in healthy controls, and in patients with calcium-containing kidney stones classified into the various calciuria types (normocalciuria, renal, absorptive, resorptive hypercalciuria). The total citrate in two subgroups (normocalciuria, renal hypercalciuria) was significantly higher than in controls. Two independent analyses showed a mea...

1. The association of varying levels of urinary pH, urinary citrate and urinary calcium and magnesium excretion rates with kidney citrate, calcium and magnesium concentrations in experimental nephrocalcinosis was examined in twenty-four rats in a 3 x 2 multifactorial experiment with four replicates. All rats received the same synthetic diet for 6 weeks before being killed in the seventh week. I...

Urinary citrate is an important inhibitor of calcium nephrolithiasis and is primarily determined by proximal tubule reabsorption. The major transporter to reabsorb citrate is Na(+)-dicarboxylate cotransporter (NaDC1), which transports dicarboxylates, including the divalent form of citrate. We previously found that opossum kidney (OK) proximal tubule cells variably express either divalent or tri...

PURPOSE The occurrence of brushite stones has increased during recent years. However, the pathogenic factors driving the development of brushite stones remain unclear. METHODS Twenty-eight brushite stone formers and 28 age-, sex- and BMI-matched healthy individuals were enrolled in this case-control study. Anthropometric, clinical, 24 h urinary parameters and dietary intake from 7-day weighed...

BACKGROUND AND OBJECTIVES Lower urinary citrate excretion is a risk factor for nephrolithiasis and associated with metabolic acidosis and higher prevalence of hypertension and insulin resistance. This study sought to quantify the independent predictors of urinary citrate excretion in population-based cohorts. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS A cross-sectional study of 2561 indivi...

OBJECTIVE To assess the effect of dietary potassium citrate supplementation on the urinary pH, relative supersaturation of calcium oxalate and struvite (defined as the activity product/solubility product of the substance), and concentrations of magnesium, ammonium, phosphate, citrate, calcium, and oxalate in dogs. ANIMALS 12 healthy adult dogs. PROCEDURE Canned dog food was fed to dogs for ...

To the Editor: 1. Millan A, Conte A, Garcia-Ease A, Grases F. Determination of citrate in urine by simple direct photometry [Tech Briefi. Clin Chem 1987;33:1259-60. 2. Ogawa Y, Morozumi M, Tanaka T, Yamaguchi K. Determination of urinary citrate by ion chromatography. J Urol 1986;135:178-81. 3. Kok DJ, Papapoulos SE, Bijvoet OLM. Excessive crystal agglomeration with low citrate excretion in recu...