Surfactant molecules are used as interesting tools to study the structure, function and stability of proteins. Protonation states of amino acids may be changed in the presence of surfactants. In this work, using experimental observations and molecular dynamic simulation, the effects of sodium dodecyl sulfate on the acid dissociation constants of tryptophan was examined. The acid–base equilibriu...

A high sensitive electrochemical nanostructure sensor based on graphene oxide/Fe3O4@SiO2 nanocomposite modified graphite screen printed electrode (GO/Fe3O4@SiO2/SPE) has been developed for trace analysis of acetaminophen. The electrochemical study of the modified electrode, as well as its efficiency for simultaneous voltammetric oxidation of acetaminophen and tryptophan is described. Compared w...

arthrobotrysamerospora (atcc 34468) produced glucoamylase in a semi-synthetic medium containing starch as a sole carbon source. polyacrylamide gel electrophoresis of crude glucoamylase showed three isoenzymes. they were designated as glu i, glu ii and glu iii according to their electrophoretic mobility. these iso-glucoamylases were purified by column chromatography using deae-sephadex a-50. the...

A novel carbon paste electrode modified with ionic liquid (n-hexyl-3-methylimidazolium hexafluoro phosphate) and magnetic core-shell manganese ferrite nanoparticles (MCSILCPE) was fabricated. The electrochemical study of the modified electrode, as well as its efficiency for electro-oxidation of tryptophan, is described. Cyclic voltammetry (CV), choronoamperometry (CHA) and square wave voltammet...

Quality Protein Maize (QPM) has about twice the amount of lysine and tryptophan of normal maize and hence represents an important tool of correcting its deficiency in protein quality. However, the effects of low nitrogen and drought on genetic parameters such as gene action and combining abilities of protein quantity and quality of QPM are not known. To study how these genetic parameters are af...

conclusions our present study shows that sugar beet molasses is a source of plp and l-ser; and hence, suggests the general application of the approach to produce l-trp more economically. results consequently, the potassium phosphate buffer system was considered as the suitably buffered reaction medium. the concentration of l-trp produced in reaction medium was 0.3 mm and 0.12 mm for glucose and...

background: salvia limbata is an aromatic herbaceous plant and grows widely in iran turkey and afghanistan. in the previous study, six flavones together with rosmarinic acid were isolated from the ethyl acetate and methanol extracts of s. limbata. in this report, we focused on the isolation and identification of the glycosylated sterols and the main accumulated amino acid of the species s. limb...

A high sensitive electrochemical nanostructure sensor based on graphene oxide/Fe3O4@SiO2 nanocomposite modified graphite screen printed electrode (GO/Fe3O4@SiO2/SPE) has been developed for trace analysis of acetaminophen. The electrochemical study of the modified electrode, as well as its efficiency for simultaneous voltammetric oxidation of acetaminophen and tryptophan is described. Compared w...

We investigated how changes in tryptophan pyrrolase activity and tryptophan loads affect the breakdown of tryptophan by tryptophan pyrrolase. Breakdown of tryptophan was estimated by injecting rats with [ring-ZJ%]tryptophan and measuring respiratory 14C02. We concluded, contrary to previous reports, that induction of tryptophan pyrrolase definitely will increase the rate of tryptophan breakdown...