We report a fluorometric technique for determination of albumin-titratable bilirubin in the jaundiced neonate. Although bilirubin alone has very little native fluorescence, considerable emission is observed in the presence of albumin under acid conditions. Analysis of the plasma sample alone and in the presence of excess human serum albumin solution appears to reflect the bilirubin tightly boun...

Ethylene glycol (EG) consumption is commonly employed as an experimental regimen to induce hyperoxaluria in animal models of calcium oxalate nephrolithiasis. This approach has, however, been criticized because EG overdose induces metabolic acidosis in humans. We tested the hypothesis that EG consumption (0.75% in drinking water for 4 wk) induces metabolic acidosis by comparing arterial blood ga...

An acid-titration method for the determination of bicarljonate and titratable acid in the one sample of urine is described. The method needs only one manipulation of the sample of urine, and was devised to simplify routine laboratory methodology. The accuracy and precision of the urinary bicarbonate method during routine laboratory use is comparable to that obtained by using the Van Slyke manom...

1. In six volunteers, the effect of intragastric administration of different water-soluble chemical isolates of dietary fibre on gastric secretion, acidity and emptying was studied. 2. At 30 min after administration of the test meals, the stomach contents were completely aspirated and the volume, pH, phenol red concentration, total titratable acidity and different electrolytes were measured. 3....

the physical and physiological characteristics of sarawak pineapple were studied at five different stages of growth from one to five months after anthesis. changes in fruit length, diameter, pulp color, pulp firmness, ph, total soluble solids, titratable acidity, ascorbic acid content and antioxidant activity were monitored. the sarawak pineapple exhibited a sigmoid growth pattern during fruit ...

The effects of varying extracellular pH on the rates of uptake of titratable anions by human erythrocytes under conditions of constant intracellular pH have been determined for a series of highly related anions, the phosphate "analogs." These compounds are simply substituted phosphorus oxyacids, differing in the number and acidity of titratable protons: phosphate (HPO4(2-), pKa 6.8); phosphite ...

BURNELL, JAMES M., ELIZABETH J. TEUBNER, AND DAVID P. SIMPSON. bfetabolic acidosis accompanying potasszum deprivation. Am. J. Physiol. 227(2): 329-333. 1974.-Removal of potassium, with or without substitution of an equivalent amount of sodium, from the diet of dogs causes a prompt and persistent decrease in distal H+ secretion resulting in an increase in urine pH. The sequelae to this change in...

Black currant berries contain many biochemical compounds with proven or potential human health benefits. We studied the content of total and single polyphenols, ascorbic acid, soluble sugars, and titratable acidity for two advanced selections and three cultivars of black currant at two distant locations in Sweden (south: 56°06'N; north: 65°21'N) over a 3 year period. Regression analyses reveale...

BAL, E. and S. CELIK, 2010. The effects of postharvest treatments of salicylic acid and potassium permanganate on the storage of kiwifruit. Bulg. J. Agric. Sci., 16: 576-584 Kiwifruit (Actinidia deliciosa cv. Hayward) was used to investigate the effects of salicylic acid (0.5 mM, 1 mM) and potassium permanganate treatments on fruit quality and storage life during storage for two years. After tr...

To test the hypothesis that amino acid residues in band 3 with titratable positive charges play a role in the binding of anions to the outside-facing transport site, we measured the effects of changing external pH (pH(O)) on the dissociation constant for binding of external iodide to the transport site, K(O)(I). K(O)(I) increased with increasing pH(O), and a significant increase was seen even a...