The neurotensin receptor NTSR1 binds the peptide agonist neurotensin (NTS) and signals preferentially via the Gq protein. Recently, Grisshammer and co-workers reported the crystal structure of a thermostable mutant NTSR1-GW5 with NTS bound. Understanding how the mutations thermostabilize the structure would allow efficient design of thermostable mutant GPCRs for protein purification, and subseq...

The thermostability of tyrosinase from three wild type strains of Neurospora crassa has been investigated. For this purpose a sequence comparison of two thermostable and one thermolabile tyrosinase isoenzyme was carried out. It revealed that at position 201 the thermostable enzyme forms share an aspartate residue in contrast to an asparagine residue in the thermolabile form. In addition, one of...

Protein engineering approach enables effective immobilisation of thermostable affinity proteins onto polystyrene materials.

Cellular components of thermophilic organisms (enzymes, proteins and nucleic acids) are also thermostable. Apart from high temperature they are also known to withstand denaturants of extremely acidic and alkaline conditions. Thermostable enzymes are highly specific and thus have considerable potential for many industrial applications. The use of such enzymes in maximising reactions accomplished...

Thermostable lipase from Geobacillus thermodenitrificans IBRL-nra was purified and characterized. The production of thermostable lipase from Geobacillus thermodenitrificans IBRL-nra was carried out in a shake-flask system at 65°C in cultivation medium containing; glucose 1.0% (w/v); yeast extract 1.25% (w/v); NaCl 0.45% (w/v) olive oil 0.1% (v/v) with agitation of 200 rpm for 24 hours. The extr...

A study was conducted to investigate the effects of thermostable phytase supplementation on the growth performance and nutrient digestibility of broilers for 42 d. 1-day-old Arbor Acres chicks with similar initial body weight were randomly divided into 3 treatments consisting 6 pens of 20 chicks per pen. The control group was given maize-soybean meal basal diet and the other two groups were fed...

Methods to increase temperature stability of vaccines and adjuvants are needed to reduce dependence on cold chain storage. We report herein creation and application of pVEX expression vectors to improve vaccine and adjuvant manufacture and thermostability. Defined media fermentation yields of 6g/L thermostable toll-like receptor 5 agonist flagellin were obtained using an IPTG inducible pVEX-fla...

Unusual thermostable and insensitive high-energy compounds which contain dioximes with donor–acceptor H-bond sites, were synthesized from glyoxal (40% in water) straightforward steps.

This study proposes a thermostable refractive index (RI) sensor consisting of a silica microsphere coated with a poly(methyl methacrylate) (PMMA) layer. The first-order and second-order whispering gallery modes (WGMs) of both TE and TM polarizations are considered theoretically. The layer thickness is carefully optimized to eliminate the thermal drift and enhance the RI sensitivity and detectio...

Lipases (triacylglycerol acylhydrolase, EC 3.1.1.3), catalyze the hydrolysis of long-chain triglycerides with the formation of diacylglyceride, monoglyceride, glycerol and fatty acids. Lipases are actively used in various industries which include food and diary, pharmaceuticals, organic synthesis and detergent and cosmetics. Thermostable lipases are commercially significant for their potential ...