Phyllosticta (Phyllostictaceae, Botryosphaeriales) species are widely distributed globally and constitute a diverse group of pathogenic endophytic fungi associated with broad range plant hosts. In this study, four new Phyllosticta, i.e. P. endophytica, jiangxiensis, machili, xinyuensis, were described using morphological characteristics multi-locus phylogeny based on the internal transcribed sp...

BACKGROUND Quantitative real-time reverse-transcriptase PCR (qRT-PCR) is an important technique for analyzing differences in gene expression due to its sensitivity, accuracy and specificity. However, the stability of the expression of reference genes is necessary to ensure accurate qRT-PCR assessment of expression in genes of interest. Perennial ryegrass (Lolium perenne L.) is important forage ...

One gene coding for yeast cytoplasmic elongation factor 1 alpha (EF-1 alpha) was isolated by colony hybridization using a cDNA probe prepared from purified EF-1 alpha mRNA. A recombinant plasmid, pLB1, with a 6-kilobase yeast DNA insert, was found by hybrid selection and translation experiments to carry the entire gene. The nucleotide sequence of the gene with its 5'- and 3'-flanking regions wa...

Abstract Taxonomy of Pseudohydnum gelatinosum and its sister taxa is revised via morphological data a four-gene dataset (ITS, nc LSU rDNA, TEF1 , RPB1 ). Identity P. Tremellodon pusillus re-established based on newly collected sequenced material from their type localities. alienum Europe; umbrosum temperate East Asia; cystidiatum meridianum placibile Vietnam; omnipavum North America are describ...

BACKGROUND Manipulations in Saccharomyces cerevisiae classically depend on use of auxotrophy selection markers. There are several disadvantages to this in a microbial cell factory setting: (1) auxotrophies must first be engineered in prototrophic strains, and many industrial strains are polyploid/aneuploid prototrophs (2) available strain auxotrophies must be paired with available repair plasmi...

Abstract Wilt disease with unknown etiology causes mass mortality in commercial Acacia mangium nurseries South Sumatra. This pathogen induces symptoms of chlorosis the lower leaves and develops into shoots; subsequently, plants wither die. research identifies pathogenic species causing this wilt to assess its pathogenicity or virulence. Fifteen isolates Fusarium oxysporum varying colony sizes c...

Abstract The systematic position of the enigmatically mycoparasitic genus Squamanita ( Agaricales , Basidiomycota ) together with Cystoderma Phaeolepiota Floccularia and Leucopholiota is largely unknown. Recently they were recognized as Squamanitaceae but previous studies used few DNA markers from a restricted sample taxa family lacked formal taxonomic treatment. In this study, newly generated ...

A new species, Pestalotiopsis licualacola, was isolated from grey leafspots of Licuala grandis (ruffled fan palm). It is morphologically distinct in having relatively small, greyish brown conidia (16–20 × 3–5 μm), and 1–3 short apical appendages without knobs. Phylogenetic analysis based on combination of ITS, β-tubulin and tef1 gene sequence data clearly distinguishes P. licualacola from other...

Modern taxonomic studies of Agaricomycetes rely on the integrative analyses morphology, environmental data, geographic distribution, and usually several DNA loci. However, sampling selection loci for are commonly shallow. In this study, we suggest minimal numbers necessary specimens to sample analyze in order prevent inadequate decisions species groups with minor morphological genealogical diff...

Integrative taxonomic studies of macrofungal diversity in the Brijuni National Park (Istria County, Croatia) led to discovery a second species Inocybe (Agaricales, Inocybaceae) new science. istriaca sp. nov. is described on basis morphological, ecological, and multigene phylogenetic analyses, its placement within family Inocybaceae discussed. The combination most important morphological charact...