A common characteristic for proteomic analyses is the need for extensive biochemical processing. Digital microfluidics (DMF), a technique characterized by the manipulation of discrete microdroplets (100 nL-10 microL) on an open array of electrodes, is a good match for carrying out rapid, automated solution-phase reactions. Here, we report a DMF-based method integrating several common processing...

Transposons have been used in many laboratories worldwide as a powerful tool for insertional mutagenesis to investigate gene function in bacteria (8). The technique has recently been adapted in which a transposon, such as mini-Tn5, delivers a unique identifying sequence of 40 nucleotides (1). This technique, termed signature-tagged mutagenesis (STM), is widely used in the search for virulence-a...

tRNA3Met, one of the non-initiating methionine-specific tRNAs in brewer's yeast was purified from bulk tRNA labelled with [32P]phosphate by two column chromatographic steps. The primary structure of this tRNA was determined by the usual fingerprinting technique. Analyses of the isolated nucleotides and oligonucleotides from digests with pancreatic and T1 ribonucleases were in good agreement and...

anaerobic digestion is a feasible technology to stabilize the solid waste generated inslaughterhouses obtaining significant quantities of biogas, considered as a clean and renewable fuel. this iswhy it is important to optimize the digestion process in order to eliminate organic matter and maximize theproduction of biogas. in this work, a system was developed for treating slaughterhouse solid wa...

The digestion of neodymium (NdFeB) magnets was investigated in the context recycling rare earth elements (i.e., Nd, Pr, Dy, and Tb). Among more conventional techniques (microwave digestion, open vessel alkaline fusion), focused infrared (FID) tested as a possible approach to rapidly efficiently solubilize NdFeB magnets. FID parameters were initially optimized with unmagnetized magnet powder sub...

anaerobic digestion is a feasible technology to stabilize the solid waste generated inslaughterhouses obtaining significant quantities of biogas, considered as a clean and renewable fuel. this iswhy it is important to optimize the digestion process in order to eliminate organic matter and maximize theproduction of biogas. in this work, a system was developed for treating slaughterhouse solid wa...

Signaling layer protocols specified within the Next Steps in Signaling (NSIS) framework may rely on the General Internet Signaling Transport (GIST) protocol to handle authorization. Still, the signaling layer protocol above GIST itself may require separate authorization to be performed when a node receives a request for a certain kind of service or resources. This document presents a generic mo...

Extraction of DNA from Schizosaccharomyces pombe cells is required for various uses, including templating polymerase chain reactions (PCRs), Southern blotting, library construction, and high-throughput sequencing. To purify high-quality DNA, the cell wall is removed by digestion with Zymolyase or Lyticase and the resulting spheroplasts lysed using sodium dodecyl sulfate (SDS). Cell debris, SDS,...

the digestibility and fermentation parameters of the diets containing leaf, pod and seed of the subabul tree in sheep was determined by using two steps digestion and gas production (gp) methods. the experimental diets consisted of 0.0 (control), 25, 50, 75 and 100 percent of the leaf, pod and seed of the subabul tree that were replaced with alfalfa in diets of sheep. diets containing 50 percent...