background one disadvantage of expressing heterologous proteins in escherichia coli is that the proteins are frequently expressed as insoluble inclusion bodies. to avoid this problem, heterologous proteins are typically expressed as a fusion protein. maltose binding protein (mbp) is one of the widely used partners for production of recombinant fusion proteins in e. coli. mbp is among the most e...

Background: Giardia lamblia is the most frequent human intestinal protozoan worldwide. Diagnosis of G. lamblia by microscopic examination of stool is common, but ELISA & IFA methods have been used in research and epidemiological studies recently. To produce polyclonal antibodies for designing ELISA & IFA kites molecular and genomic studies (PCR and parasitic culture) as well as other epidemiolo...

Despite significant effort to quantify the interdependence of the water and energy sectors, global requirements of energy for water (E4W) are still poorly understood, which may result in biases in projections and consequently in water and energy management and policy. This study estimates water-related energy consumption by water source, sector, and process for 14 global regions from 1973 to 20...

introduction: polyclonal antibodies are required to be affinity purified. improved purification methods of polyclonal antibody provide an opportunity to pick the most purified immunoglobulins as a primary or secondary antibody in immunoassays that are included in many vaccine studies. two common techniques for purifying proteins is salt precipitation and chromatography purification. our work fo...

one of the most important aspects in recombinant biologic production, based on gmp rules, is the accuracy of final product quality control, especially assessment of host cell macromolecules contamination rate in final product. the purification requirement can be eliminated when the yeast cell containing the recombinant protein is used as a host cell. it is possibile that the final product conta...

Novel magnetic biochar has been successfully synthesized by using microwave technique, using discarded materials such as Empty Fruit Bunch (EFB). The optimized conditions for the best novel magnetic biochar synthesis are at 900 w reaction power, 20 min reaction time, and impregnation ratio 0.5 (biomas:FeCl3) The details physical and chemical analyses of novel magnetic biochar wer...

growing applications of phenylalanine dehydrogenase (phedh) enzyme in the medical and pharmaceutical industries encourages researchers to seek simple, fast and economical alternative purification methods. with goal of finding a new technique, the extraction and purification of recombinant bacillus badius phedh in polyethylene glycol 6000 (peg-6000) and ammonium sulfate aqueous two-phase systems...