In studying formation of an arsenic-lipid complex during the active transport of (74)As-arsenate in yeast, it was found that adaptation of yeast to arsenate resulted in cell populations which showed a deficient inflow of arsenate as compared to the nonadapted yeast. Experiments with both types of cells showed a direct correlation between the arsenate taken up and the amount of As-lipid complex ...

In studying formation of an arsenic-lipid complex during the active transport of 74As-arsenate in yeast, it was found that adaptation of yeast to arsenate resulted in cell populations which showed a deficient inflow of arsenate as compared to the nonadapted yeast. Experiments with both types of cells showed a direct correlation between the arsenate taken up and the amount of As-lipid complex fo...

Initial experiments involving mouse development employed single IP injections of 45 mg/kg sodium arsenate on one of days 6-12 of gestation and produced a spectrum of developmental defects. Embryotoxicity was indicated by high prenatal mortality and decreased fetal weights. A chelating agent, 2,3-dimercaptopropanol (BAL), was then employed in an attempt to alleviate the adverse effects of prenat...

A series of copper(II), nickel(II) and zinc(II) dimetallic complexes were prepared and their affinities towards arsenate investigated. Indicator displacement assays (IDAs) were carried out to establish the complexes with best affinities towards arsenate. A di-zinc complex (3) was selected and its arsenate-binding abilities investigated by isothermal titration calorimetry (ITC). The X-ray crysta...

We have used yeast Saccharomyces cerevisiae as a tool to identify the importance of mitochondrial processes involved in arsenic-induced carcinogenicity in humans. We screened 466 single-gene knockout strains of yeast S. cerevisiae known to be involved in biogenesis of mitochondria for sodium arsenite (AsIII) and sodium arsenate (AsV) sensitivity. We identified 72 arsenite-sensitive and 81 arsen...

Effect of sodium arsenate (Na2HAsO4.7H2O) on the growth and activity of few oxidizing enzymes of mungbean seedlings was investigated and it was found to have drastic effects on the growth and biochemical metabolism of germinating mungbean (Vigna radiata (L.) Wilczek cv. B-105) seedlings. With the increase in concentration of sodium arsenate (5μM, 10μM and 20μM) significant decrease in seedling ...

The developmental origin of arsenate-induced renal agenesis was investigated. Pregnant Wistar rats were each injected once ip with 45 mgkg sodium arsenate a t day 10 (sperm day = day 0). Pregnancy was terminated a t various times following injection and the embryos recovered and serially sectioned. Renal agenesis resulted when the mesonephric duct failed to give rise to a ureteric bud with subs...