Effect of Cryopreservation Protocol on Post-Thaw Characteristics of Stallion Spermatozoa. (August 2009) Jose L. Salazar, B.S., Texas A&M University Chair of Advisory Committee: Dr. Dickson D. Varner Three ejaculates from each of eight stallions were initially centrifuged in INRA 96 extender and spermatozoal pellets were resuspended in a milk/egg yolk-based freezing extender or an egg yolk-based...

This work was initiated to study the concentration effects on phenanthrene and pyrene extraction during process of aging in phaeozem and burozem with or without freeze-thawing cycles. 1, 10, and 100 μg g(-1) phenanthrene and pyrene contaminated soils were extracted by 10,000 mg L(-1) surfactant SDBS at various times. The extraction amount decreased with increasing contact time. Aging process co...

To examine effects of varied freezing conditions on the development of spatial heterogeneity in the frozen protein solution, macroscopic freeze concentration and micro-segregation of bovine serum albumin (BSA) were investigated in a temperature-controlled 200-ml freeze container. Freezing to -40 °C promoted formation of protein concentration gradients (69-114 μg ml-1) in frozen samples taken fr...

In this article we show that in the usual type-I seesaw framework, augmented solely by a neutrino portal interaction, dark matter (DM) relic density can be created through freeze-in, manner fully determined interactions and DM particle mass. This simple freeze-in scenario, where is not state, proceeds slow, seesaw-induced decays of Higgs $W$ $Z$ bosons. We identify two scenarios, one which pred...

OBJECTIVE To report a live birth after transfer of cryopreserved pronuclear embryos in cryostorage for almost 20 years. DESIGN Case report. SETTING Academic IVF center. PATIENT(S) A 42-year-old female patient with low ovarian reserve receiving donated embryos. These embryos were the result of an anonymous donation from an infertile couple who had conceived during her IVF treatment. INTE...

Background: The slow-freezing method is one of the most popular techniques to freeze human oocytes and it is also used in routine in vitro-fertilization (IVF) programs, particularly in those countries where embryo freezing is not allowed. Slow-freezing damages some of the structures of the egg, among which the meiotic spindle (MS) and the zona pellucida (ZP). Polarized Light Microscopy (PLM) pe...

The freezing of aqueous solutions and reciprocal distribution of ice and a freeze-concentrated solution (FCS) are poorly understood in spite of their importance in fields ranging from biotechnology and life sciences to geophysics and climate change. Using an optical cryo-microscope and differential scanning calorimetry, we demonstrate that upon cooling of citric acid and sucrose solutions a fas...

Radicular cysts are the most common cystic lesions affecting the jaws. They are most commonly found at the apices of the involved teeth. This condition is usually asymptomatic but can result in a slow-growth tumefaction in the affected region. The following case report presents the successful treatment of radicular cysts using autologous periosteum and platelet-rich fibrin with demineralized fr...