(Running title): overview of silver staining methods for proteins ABSTRACT On the basis of the physico-chemical principles underlying silver staining of proteins, which are recalled in this paper, several methods of silver staining of proteins after SDS electrophoresis in polyacrylamide gels or isoelectric focusing were tested. The most valuable protocols are presented in this report, including...

CS preparations for topical and/or oral use were subsequently developed to overcome the astringency of, and staining by, simple silver salts[2] while retaining the long-known antiseptic properties of metallic silver.[3] The older preparations, pre-1910, usually contained high levels of oxidized silver (Ag+), not precipitated by isotonic salt solutions, made from silver nitrate and various carri...

An improved method, which exhibited simultaneously clearly kinetochores and the nucleolar organizer regions of human chromosomes by pretreating of human metaphase chromosomes with HCl and NaOH, followed by staining with silver nitrate and visualizing using ammoniacal silver, is described in the present communication. It has important role for analysis of kinetochore variation, mechanism of chro...

The Golgi-Cox is a gold standard technique to stain the neurons. However the major limitations of the study are that it is time taking and inconsistent. In this paper we describe a modification of the Golgi–Cox method, in which the tissue blocks were immersed in rapid Golgi solution and silver nitrate for staining of neurons in rat brain. The method is simple, reproducible, rapid, inexpensive a...

On the basis of the physico-chemical principles underlying silver-staining of proteins, which are recalled in this paper, several methods of silver-staining of proteins after SDS electrophoresis in polyacrylamide gels or isoelectric focusing were tested. The most valuable protocols are presented in this report, including standard methods for unsupported gels and new methods devised for thin (0....

A running colour electrophoresis of protein in sodium dodecylsulfate (SDS)-polyacrylamide gel (PAG) has been developed and the coloured gel was restained with silver nitrate and hydroquinone (HQ) within 20min. Trichloroacetic acid (TCA) was found to be a powerful destaining reagent and reproducibility of this double staining procedure was excellent.

What is the difference between argentaffin and argyrophilic reactions? Both terms relate to the formation of colloidal metallic silver (latin argentum, Greek argyros) (1), which may range in color from yellow through brown to black depending on particle size and density, in specific structural components of tissues. the metal is formed at sites of reduction of silver ions derived from a stainin...

Objective: To synthesize silver nanoparticles extracellularly using Bacillus sp. isolated from soil sample. Methods: Bacteria capable of synthesizing silver nanoparticles extracellularly were screened by treating culture supernatant with silver nitrate solution. The potential isolate was isolated on nutrient agar medium and identified by Gram staining and biochemical tests. Results: One of the ...

Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-subme...

AIM To evaluate whether lead nitrate could replace uranyl nitrate in the Genta stain for H pylori without sacrificing the advantages of the triple stain (Steiner silver impregnation combined with Alcian blue and haematoxylin/eosin (H&E)). METHODS A comparison was made in 16 specimens between the original triple stain and the revised version. One pathologist evaluated all sections. RESULTS D...