Mussels Mytilus galloprovincialis collected and bought from different locations (Slovenian coastline, Italy and NE Pacific) were analysed by techniques of hydride generation atomic fluorescence spectrometry (HG-AFS) and liquid chromatography coupled to inductively coupled plasma mass spectrometry (HPLC-ICP-MS) in order to assess selenium (Se), its distribution and Se species in raw and cooked s...

A sensitive and robust method for the determination of five inorganic and organic Se species in human urine by reversed-phase liquid chromatography with mixed ion-pair reagents coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) is described in this paper. A good separation for anionic, cationic and neutral Se species, namely selenate [Se(VI)], selenourea (SeUr), selenomethi...

For extracellular vesicle research, whether for biomarker discoveries or therapeutic applications, it is critical to have high-quality samples. Both microscopy and NanoSight Tracking Analysis (NTA) for size distribution have been used to detect large vesicles. However, there is currently no well-established method that is convenient for routine quality analysis of small-size impurities in vesic...

The synthesis of D- and L-selenomethionine labeled with ⁸²Se and three deuteriums at Se-methyl group (D- and L-[²H₃, ⁸²Se]selenomethionine) was described. D- And L-[²H₃, ⁸²Se]selenomethionine were prepared by condensation of (R)- and (S)-2-amino-4-bromobutylic acid with lithium [²H₃, ⁸²Se]methaneselenolate, which was prepared from metal (82)Se and [²H₃]methyl iodide. The optical purities of D- ...

two modified methods for assaying sodium diclofenac were developed by gc and hplc. diclofenac was converted into methyl ester derivative by methyl iodide in acetone. the ester was extract and subjected to glc with flame ionization detector. %5 se-30/chrom w-hp (80-100 mesh) was used as a column in gc. for reversed phase hplc, the mobile phase was methanol and water (55-45). the separation was p...

A method for the quantitation of proteinaceous selenocysteine (SeCys) in Se-rich yeast was developed. The method is based on the reduction of the Se-Se and S-Se bridges with dithiotretiol, derivatization with iodoacetamide (carbamidomethylation), followed by HPLC-ICP MS. The chromatographic conditions were optimized for the total recovery of the proteinaceous selenocysteine, the minimum number ...

Increasing speciation demands in clinical chemistry, toxicology and nutrition have made the determination of the total elements in a sample inadequate; the amount of an element and the chemical forms in which it is present need to be known. Inductively coupled plasma mass spectrometry (ICP-MS) was used after high-performance liquid chromatographic (HPLC) separation, as was electrospray ionizati...

The high mobility group (HMG) of nonhistone proteins have been investigated using two high performance liquid chromatographic techniques (HPLC). Reversed-phase HPLC under conditions of 50 mM triethylamine adjusted to pH 2.2 with phosphoric acid (solvent A) and 95% acetonitrile in water (solvent B) was used to separate proteins primarily on the basis of differences in the overall hydrophobi...