Secretory diarrhea remains a global health burden and causes major mortality in children. There have been some focuses on antidiarrheal therapies that may reduce fluid losses and intestinal motility in diarrheal diseases. In the present study, we identified shikonin as an inhibitor of TMEM16A chloride channel activity using cell-based fluorescent-quenching assay. The IC50 value of shikonin was ...

Rotaviral gastroenteritis is a serious public health problem in both developed and developing countries. The disease is ubiquitous, affecting nearly all children by the age of 5 years. It is the most common cause of hospitalizations for gastroenteritis among children in the United States (30%-70% depending on the season) and is associated with direct and indirect costs of approximately $1 billi...

introduction: in developing countries, rotaviral diarrhea is the cause of death of many children, and is the main etiology of gastroenteritis in children under 2 years old. determination of the prevalence of rotaviral infection can reveal the impact of this infection in this group of the population and provides clues as to the strategies for prevention and treatment protocols for children suffe...

Rotavirus gastroenteritis is one of the main causes of acute diarrhea in young humans and animals worldwide. The colostrum-deprived, artificially-reared, neonatal pig has been extensively used in our laboratory as a model animal for studying an experimentally-induced rotaviral gastroenteritis. Details on procurement of newborn pigs, immunological characteristics and artificial rearing condition...

Rotavirus gastroenteritis is one of the main causes of acute diarrhea in young humans and animals worldwide. The colostrum-deprived, artificially-reared, neonatal pig has been extensively used in our laboratory as a model animal for studying an experimentally-induced rotaviral gastroenteritis. Details on procurement of newborn pigs, immunological characteristics and artificial rearing condition...

Rotaviral diarrhea is endemic in most areas of the world, yet community-wide epidemics have not been reported in prospectively monitored populations. This prospective study of the etiology of diarrhea included biweekly visits to the homes of 10% of the population of the White Mountain Apache Indians and began in April 1981. During a three-week period beginning 21 October, 1981, 342 new cases of...

a rapid and simple technique for diagnosis of rotaviral diarrhea is polyacrylamide gel electrophoresis (page) of dsrna extracted from fecal samples. to determine the electrophoretype of iranian calf rotavirus, 23 rotaviral positive fecal samples from diarrheic holstein calves at the age of less than one month in tehran region were examined. the viral dsrna was extracted with phenol-chloroform, ...

Three rare human G12 strains were detected from diarrheic clinical samples of children (<8 months of age) in Calcutta during a routine surveillance study of rotaviral diarrhea in India. The VP7 genes of G12 strains and their products showed maximum homology (97 to 99% at the nucleotide level and 98% at the amino acid level, respectively) with those of two recently reported G12 strains (from the...

OBJECTIVE To extend a nation-wide rotavirus surveillance network in India, and to generate geographically representative data on rotaviral disease burden and prevalent strains. DESIGN Hospital-based surveillance. SETTING A comprehensive multicenter, multi-state hospital based surveillance network was established in a phased manner involving 28 hospital sites across 17 states and two union t...